Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
AssigneeEpimeron USA, Inc.
InventorsTrish Choudhary, Xueyang Feng, Thanh Nguyen, Matthew Workentine
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Abstract
The present disclosure relates to recombinant polypeptides that have olivetolic acid cyclase activity, nucleic acids encoding these recombinant polypeptides, recombinant host cells that produce these recombinant polypeptides, and compositions comprising the recombinant polypeptides, nucleic acids, and/or recombinant host cells. The present disclosure also relates to uses of these recombinant polypeptides, nucleic acids encoding them, and recombinant host cells comprising them, in methods for the preparation of cannabinoids and cannabinoid precursors.
Claims18
A recombinant polypeptide having olivetolic acid cyclase activity, wherein the polypeptide comprises an amino acid sequence of at least 90% identity to SEQ ID NO:
- 6, and an amino acid residue difference as compared to SEQ ID NO:
- 6 at each of a combination of six positions, wherein the combination of six positions are selected from V8, K49, 158, E64, T68, and 174, and the amino acid residue differences at the positions are selected from:
- V8I, K49A, K49C, K49G, K49H, K49L, K49N, K49P, K49R, K49S, K49T, K49V, 158C, I58V, E64D, E64K, T68A, T68C, T68E, T68G, T68H, T68M, T68Q, T68S, 174G, 174H, I74K, I74L, 174M, 174N, 174Q, I74R, I74S, I74T, and I74V.
Dependencies are inferred from the claim text.