Modified terpene synthases and their use for production of pseudopterosin intermediates and/or pseudopterosins

A modified terpene synthase comprising at least one modified amino acid residue as compared to an amino acid sequence corresponding to an unmodified wild type terpene synthase according to SEQ ID NO:

• 1, wherein said at least one modified amino acid residue is located in an α-helix structure being part of, or close to, an active site pocket of the terpene synthase, and wherein said at least one modified amino acid residue is an amino acid with a hydrophobic side chain and/or an amino acid with a polar uncharged side chain;
• wherein the modified terpene synthase has at least 95% sequence identity to the unmodified wild type terpene synthase according to SEQ ID NO:
• 1;
• wherein said at least one modified amino acid residue is a substitution of a wild type amino acid residue selected from:
• (i) methionine at position 71,
• (ii) methionine at position 75,
• (iii) glycine at position 182,
• (iv) histidine at position 184,
• (v) methionine at position 300, and
• (vi) methionine at position 304,
• in the amino acid sequence of the unmodified wild type terpene synthase according to SEQ ID NO:
• 1.