Mutations for improving activity and thermostability of PETASE enzymes
AssigneeBoard of Regents, The University of Texas System
InventorsHongyuan Lu, Daniel Diaz, Hannah Cole, Raghav Shroff, Andrew Ellington, Hal Alper
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Abstract
This invention relates to engineered plastic-degrading enzymes with improved functional features. In particular, modified PETases capable of hydrolysis of PET or polyethelene terephthalate plastic polymer are disclosed herein. These engineered PETases contain one or more amino acid modifications at specified residue or residues, for example, N233, and exhibit improved enzymatic activity as well as thermostability.
Claims18
An engineered PET (poly(ethylene terephthalate)) hydrolase comprising an amino acid sequence at least 90% identical to SEQ ID NO:1 and having at least one mutation corresponding to N233 relative to SEQ ID NO:1.
Dependencies are inferred from the claim text.