Acetyl-transferases

A modified acetyl transferase enzyme comprising an amino acid sequence having at least 90% identity to SEQ ID NO:1, wherein:

• the amino acid sequence comprises one or more substitute residue(s) relative to SEQ ID NO:1,
• the one or more substitute residue(s) are located at position(s) corresponding to position 68, 69, 72, 73, 171, 172, 173, 174, 176, 178, 291, 292, 294, 301, 307, 308, 311, 312, 320, 322, 334, 362, 405, 407, 409, 480, 483, 484, 490, 492, 520, 521, 522, 524, 525, 526 and combinations thereof according to SEQ ID NO:1, and
• the one or more substitute residue(s) is/are selected from the group consisting of:
• substitute residue corresponding to position 68 according to SEQ ID NO:1 is threonine, substitute residue corresponding to position 69 according to SEQ ID NO:1 is asparagine or serine or alanine, substitute residue corresponding to position 72 according to SEQ ID NO:1 is asparagine or lysine, substitute residue corresponding to position 73 according to SEQ ID NO:1 is leucine, substitute residue corresponding to position 171 according to SEQ ID NO:1 is lysine or asparagine, substitute residue corresponding to position 172 according to SEQ ID NO:1 is glycine, substitute residue corresponding to position 173 according to SEQ ID NO:
• 1 is leucine, substitute residue corresponding to position 174 according to SEQ ID NO:1 is isoleucine, substitute residue corresponding to position 176 according to SEQ ID NO:1 is alanine or glycine, substitute residue corresponding to position 178 according to SEQ ID NO:1 is valine, substitute residue corresponding to position 291 according to SEQ ID NO:1 is serine or glycine, substitute residue corresponding to position 292 according to SEQ ID NO:1 is alanine or serine or asparagine, substitute residue corresponding to position 294 according to SEQ ID NO:1 is leucine or valine, substitute residue corresponding to position 301 according to SEQ ID NO:1 is phenylalanine, substitute residue corresponding to position 307 according to SEQ ID NO:1 is isoleucine, substitute residue corresponding to position 308 according to SEQ ID NO:1 is valine, substitute residue corresponding to position 311 according to SEQ ID NO:1 is methionine or isoleucine, substitute residue corresponding to position 312 according to SEQ ID NO:1 is alanine, substitute residue corresponding to position 320 according to SEQ ID NO:1 is asparagine, substitute residue corresponding to position 322 according to SEQ ID NO:1 is valine or phenylalanine, substitute residue corresponding to position 334 according to SEQ ID NO:1 is methionine or leucine, substitute residue corresponding to position 362 according to SEQ ID NO:1 is alanine, substitute residue corresponding to position 405 according to SEQ ID NO:1 is alanine, substitute residue corresponding to position 407 according to SEQ ID NO:1 is isoleucine, substitute residue corresponding to position 409 according to SEQ ID NO:1 is alanine, substitute residue corresponding to position 480 according to SEQ ID NO:1 is glutamic acid or leucine or methionine or phenylalanine or glutamine, substitute residue corresponding to position 483 according to SEQ ID NO:1 is valine, substitute residue corresponding to position 484 according to SEQ ID NO:1 is leucine, substitute residue corresponding to position 490 according to SEQ ID NO:1 is isoleucine, substitute residue corresponding to position 492 according to SEQ ID NO:1 is valine, substitute residue corresponding to position 520 according to SEQ ID NO:1 is alanine, substitute residue corresponding to position 521 according to SEQ ID NO:1 is alanine or valine, substitute residue corresponding to position 522 according to SEQ ID NO:1 is serine or threonine, substitute residue corresponding to position 524 according to SEQ ID NO:1 is asparagine or serine, substitute residue corresponding to position 525 according to SEQ ID NO:1 is valine or isoleucine or arginine, and substitute residue corresponding to position 526 according to SEQ ID NO:1 is serine, and combinations thereof.