Anti-CD40 binding molecules and bi-specific antibodies comprising such

A humanized antibody specific to human CD40, wherein the humanized antibody comprises a heavy chain variable region (VH) and a light chain variable region (VL), wherein:

• (i) the VH comprises a framework of IGHV3-73*01 and heavy chain complementary determining regions (CDRs) 1, 2, and 3, and the VL comprises a framework of IGKV1-39*01 and light chain CDRs 1, 2, and 3;
• wherein
• the VH comprises the heavy chain CDR1 comprising the amino acid sequence of GFNFNDSFMN (SEQ ID NO:1), GENFQDSFMN (SEQ ID NO:2), GENFNDAFMN (SEQ ID NO:
• 3), or GENENDYFMN (SEQ ID NO:4), the heavy chain CDR2 comprising the amino acid sequence of QIRNKNYNYATYYTESLEG (SEQ ID NO:5), and the heavy chain CDR3 comprising the amino acid sequence of YYYDGFADYFDY (SEQ ID NO:6);
• and the VL comprises the light chain CDR1, light chain CDR2, and light chain CDRs comprising the amino acid sequences of KASQNIYIYLN (SEQ ID NO:7), NTNNLOT (SEQ ID NO:8), and LOHSSRRT (SEQ ID NO:9), respectively;
• or
• (ii) the VH comprises a framework of IGHV3-23*04 and heavy chain CDRs 1, 2, and 3, and the VL comprises a framework of IGKV1-39*01 and light chain CDRs 1, 2, and 3;
• wherein:
• the VH comprises the heavy chain CDR1, the heavy chain CDR2, and the heavy chain CDR3 comprising the amino acid sequences of GFTFTNYGLH (SEQ ID NO:16), SISPSGGVTYYRDSVKG (SEQ ID NO:17), and PFLGWGGANWIAH (SEQ ID NO:18) respectively;
• and the VL comprises the light chain CDR1, the light chain CDR2, and the light chain CDR3 comprising the amino acid sequences of LASEDISNDLA (SEQ ID NO:19), FVDRLLD (SEQ ID NO:20), and QQSYKYPPT (SEQ ID NO:21), respectively.