Methods to block aphid transmission of poleroviruses and to develop virus management tools
AssigneesThe United States of America as represented by the Secretary of Agriculture, Cornell University
InventorsMichelle L. Heck, Jennifer R. Wilson, Carl J. Schiltz, Joshua S. Chappie, Myfanwy C. Adams
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Abstract
Disclosed herein are plant-based, molecular and diagnostic tools that can be used to block aphid transmission of poleroviruses, including stabilized proteins for expression in transgenic plants and/or in formulations for direct plant delivery. Further disclosed are proteins that can kill aphids and methods to produce these proteins. Antibodies and useful for diagnostic and therapeutic uses against poleroviruses.
Claims18
An isolated protein comprising the protein sequence of SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 12.
A vector comprising a nucleic acid encoding a protein having SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 12.
A recombinant potato leaf roll virus comprising the protein of SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 12.
A modified protein comprising a polypeptide having at least 98% identity to SEQ ID NO:
- 2 or 98% identity to SEQ ID NO:
- 3, and wherein the modified protein comprises an amino acid substitution in any one of residues 137, 138, 139, 140, 141, 142, 143, 144, or 145.
A transgenic plant comprising a heterologous nucleic acid encoding SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 12.
A plant comprising a protein having the amino acid sequence of SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 12.
A method of controlling aphids, comprising the step of:
- exposing an aphid to a protein having an amino acid sequence:
- 1) at least 98% identical to SEQ ID NO:
- 2 and wherein the protein comprises an amino acid substitution in at least one of residues 137, 138, 139, 140, 141, 142, 143, 144, or 145, and the protein further comprises a green-fluorescent protein (GFP), a yellow fluorescent protein (YFP), strep tag, FlAsH tag, or polyhistidine (HIS) tag operably linked to the protein;
- or 2) at least 98% identical to SEQ ID NO:
- 3 and wherein the protein comprises an amino acid substitution in any one of residues 137, 138, 139, 140, 141, 142, 143, 144, or 145, and the protein further comprises a green-fluorescent protein (GFP), a yellow fluorescent protein (YFP), strep tag, FlAsH tag, or polyhistidine (HIS) tag operably linked to the protein, thereby inducing increased mortality in the aphid.
Dependencies are inferred from the claim text.