Point of need diagnostic with thermal control
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorJennifer Blain Christen
Life of this patent
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Abstract
Provided herein are systems and methods for a point of need testing system. In some embodiments, the point of need testing system includes a first collector and a sample amplifier. The first collector is configured to collect a first biofluid sample. The sample amplifier includes an inlet dimensioned to receive the first biofluid sample. The sample amplifier further includes a filter, a heater, and a second collector. In some embodiments, a reader can be used with the sample amplifier to read a second biofluid produced by the sample amplifier.
Claims11
A point of need testing system comprising:
- a first collector configured to collect a first biofluid sample;
- a sample amplifier configured for performing an amplification reaction to generate amplification products of a target nucleic acid to produce a second biofluid sample, the sample amplifier comprising:
- a first inlet;
- wherein the sample amplifier is fluidly coupled to the first collector by the first inlet,
- wherein the first inlet is dimensioned to receive the first biofluid sample from the first collector;
- a reagent reservoir containing one or more reagents for performing the amplification reaction to generate amplification products of the target nucleic acid;
- a filter fluidly coupled to the first collector and to the reagent reservoir, and configured to remove particulates from the first biofluid sample;
- an amplifier heater positioned adjacent to the reagent reservoir and configured to accelerate reaction kinetics of the amplification reaction of the target nucleic acid within the first biofluid sample to produce the second biofluid sample;
- a sample amplifier housing,
- wherein the filter, the reagent reservoir, and the amplifier heater are disposed within the sample amplifier housing,
- a second collector removably coupled to the reagent reservoir and configured to collect the second biofluid sample;
- an excitation array;
- a photodetector;
- and
- a reader configured to accept the second biofluid sample and produce an output, the reader comprising:
- a second inlet dimensioned to receive the second biofluid sample from the second biofluid collector;
- a lateral flow test strip coupled to the second inlet and configured to transport the second biofluid sample from the second inlet to a detection site positioned between the excitation array and the photodetector;
- wherein the excitation array is arranged to irradiate the detection site and the photodetector is arranged to detect electromagnetic radiation emitted from the detection site and configured to produce the output;
- a reader heater positioned adjacent to the lateral flow test strip configured to heat the second biofluid sample transporting along the lateral flow test strip;
- and
- a reader housing, wherein the lateral flow test strip, the detection site, and the reader heater are disposed within the reader housing.
Dependencies are inferred from the claim text.