PBMC separation systems and methods of use

A fluidic cartridge for separation of blood by rotational force, the fluidic cartridge comprising:

• an inlet region comprising a wash reservoir and a sample reservoir having an opening adapted to receive a blood sample;
• a separation region comprising a separation reservoir and a density gradient media reservoir comprising a medium for separation of blood components upon application of rotational force;
• a collection region comprising a plasma collection reservoir, and a PBMC collection reservoir;
• a plurality of channels connecting the sample reservoir, the plasma collection reservoir, and the density gradient media reservoir to the separation reservoir, and connecting the PBMC collection reservoir, the wash reservoir, a storage media reservoir, and a waste collection reservoir to the sample reservoir;
• and
• a plurality of valves wherein a valve of the plurality of valves is on each channel of the plurality of channels, wherein each valve of the plurality of valves comprises an empty valve cavity and a valve cover, said valve cover bonded to a top portion of the fluidic cartridge that is adhered to a bottom portion of the fluidic cartridge, said valve cover having a rigid portion and a flexible portion that forms a continuous seal above the valve cavity,
• wherein the top portion of the fluidic cartridge has two top portion openings at each valve to form a passage from a fluidic channel through the valve cavity when the valve is in an open position,
• wherein the valve is configured to be opened and closed repeatedly, and
• wherein the valve is not a wax valve.

A fluidic cartridge for separation of blood by rotational force, the fluidic cartridge comprising:

• an inlet region comprising a wash reservoir and a sample reservoir having an opening adapted to receive a blood sample;
• a separation region comprising a separation reservoir and a density gradient media reservoir comprising a medium for separation of blood components upon application of rotational force;
• a collection region comprising a plasma collection reservoir, and a PBMC collection reservoir;
• a plurality of channels connecting the plasma collection reservoir and the density gradient media reservoir to the separation reservoir, connecting the PBMC collection reservoir, the wash reservoir, a storage media reservoir, and a waste collection reservoir to the sample reservoir, and connecting the outer edge of the sample reservoir to the inner edge of the separation reservoir;
• and
• a plurality of valves wherein a valve of the plurality of valves is on each channel of the plurality of channels, wherein each valve of the plurality of valves comprises an empty valve cavity and a valve cover, said valve cover bonded to a bottom portion of the fluidic cartridge that is adhered to a top portion of the fluidic cartridge, said valve cover having a rigid portion and a flexible portion comprising a flexible membrane that forms a continuous seal beneath the valve cavity,
• wherein the bottom portion of the fluidic cartridge has two bottom portion openings at each valve to form a passage from a fluidic channel through the valve cavity when the valve is in an open position,
• wherein the valve is configured to be opened and closed repeatedly,
• wherein the valve is not a wax valve, and
• wherein the fluidic cartridge comprises a plurality of capillary stop valves connected to the plurality of channels to prevent fluidic movement through the fluidic cartridge without centrifugal force.