Back to results
US 12,661,647 B2Granted
Methods for enclosing and analyzing a cell in a fluidic device
AssigneeCellanome, Inc.
InventorsTarun Kumar Khurana, Ali Agah, Yir-Shyuan Wu, Pier Federico Gherardini, Filiz Gorpe Yasar
Life of this patent
Estimating the term…
Abstract
Described herein are systems and methods for analyzing biological samples (e.g., cells), including a method comprising (a) introducing a first cell into a fluidic device, (b) introducing a second cell into the fluidic device, (c) introducing a polymer precursor into the fluidic device, (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in the fluidic device.
Claims46
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in the fluidic device;
- and
- (e) measuring cytotoxicity of the first cell against the second cell.
3 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in the fluidic device;
- and
- (e) detecting a protein secreted by the first cell, the second cell, or the first cell and the second cell, wherein the detecting comprises binding the protein to a protein affinity reagent coupled to a protein-capture surface, and wherein in (d), the first cell and the second cell are selectively enclosed within a chamber comprising a polymer matrix wall, and wherein the protein-capture surface is disposed within the chamber.
3 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in the fluidic device;
- and
- (e) detecting a protein secreted by the first cell, wherein the protein secreted by the first cell is an interferon-gamma (IFN-g), an interferon-alpha (IFN-a), an interleukin, a colony stimulating factor (CSF), a tumor necrosis factor (TNF), or an effector molecule.
3 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- and
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in the fluidic device,
- wherein the introducing of the first cell in (a), the introducing of the second cell in (b), and the introducing of the polymer precursor in (c) are performed at the same time.
3 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- (d) introducing a plurality of protein-capture surfaces each coupled to a plurality of protein affinity reagents into the fluidic device, wherein the plurality of protein affinity reagents are configured to bind to a plurality of proteins secreted by the first cell;
- (e) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively co-enclosing (i) the first cell, (ii) the second cell, and (iii) at least a protein-capture surface of the plurality of protein-capture surfaces in a chamber comprising a polymer matrix wall in the fluidic device;
- and
- (f) measuring binding of the plurality of proteins secreted by the first cell to the plurality of protein affinity reagents, thereby detecting the plurality of proteins secreted by the first cell.
4 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in the fluidic device;
- and
- (e) detecting activation of the first cell.
3 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in a chamber comprising a polymer matrix wall in the fluidic device;
- and
- (e) removing an additional cell that is not enclosed by the chamber from the fluidic device.
3 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- and
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in a chamber comprising a polymer matrix wall in the fluidic device, wherein the polymer matrix wall comprises a hydrogel.
2 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- and
- (d) using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in a chamber comprising a polymer matrix wall in the fluidic device;
- and
- (e) at least partially degrading the chamber.
4 more elements
A method, comprising:
- (a) introducing a first cell into a fluidic device;
- (b) introducing a second cell into the fluidic device;
- (c) introducing a polymer precursor into the fluidic device;
- (d) determining a location of at least the first cell within the fluidic device;
- and
- (e) subsequent to (d), using a virtual photomask to selectively apply light to the fluidic device to polymerize the polymer precursor, thereby selectively enclosing the first cell and the second cell in the fluidic device.
3 more elements
Dependencies are inferred from the claim text.