Use of low pH active alpha-1,4;/1,6-glycoside hydrolases (GLCH) as a feed additive for ruminants to enhance starch digestion
AssigneeInternational N&H Denmark Aps
InventorsShukun Yu, Karsten Matthias Kragh, Wenting Li
Life of this patent
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Abstract
Disclosed are uses of at least one alpha-1,4/1,6-glycoside hydrolase (GLCH) as a feed additive for a ruminant wherein said hydrolase: (a) has at least 20% activity at pH less than or equal to 3 in the presence of pepsin as compared to activity of the hydrolase at pH 6 in the presence of pepsin, (b) said hydrolase is active in at least two of three digestive chambers of a ruminant comprising a rumen, an abomasum and a small intestine and (c) the hydrolase works with pancreatic amylase to increase glucose yield.
Claims9
A method for increasing starch digestibility and glucose yield in a ruminant which comprises:
- (a) adding at least one fungal glucoamylase as a feed additive to feed for a ruminant,
- wherein the at least one fungal glucoamylase is an EC 3.2.1.3 enzyme derived from Trichoderma reesei and:
- (i) has at least 20% activity at pH less than or equal to 3 in the presence of pepsin as compared to activity of the hydrolase at pH 6 in the presence of pepsin;
- (ii) is active in at least two of the three digestive chambers of a ruminant comprising a rumen, an abomasum, and a small intestine;
- (iii) works with digestive enzymes present in the digestive chambers of the ruminant to increase starch digestibility and glucose yield;
- and
- (iv) has at least 20% catalytic activity on maltose;
- and
- (b) administering said feed additive to the ruminant, wherein starch digestibility and glucose yield in the ruminant is increased by said addition.
Dependencies are inferred from the claim text.