Optimized cell transplant, and preparation method and use thereof

A preparation method of an optimized cell transplant, for preparing an optimized cell transplant with high survival rate and simultaneous high expression of multiple genes;

• the preparation method comprising the following steps:
• a cell modified culture step:
• mesenchymal stem cells are cultured in a first medium at a cell density of 6,000-15,000 cells/cm2, wherein the surface of a culture dish used has an oxygen-containing functional group and thus features a net negative surface charge due and hydrophilic;
• the first medium is removed after a first culture time to obtain a modified cell intermediate;
• and
• a cell optimized culture step:
• the modified cell intermediate is cultured in a second medium, and the second medium is removed after a second culture time to obtain an optimized cell transplant, wherein
• the mesenchymal stem cells are any one selected from adipose-derived stem cells, bone marrow stem cells, peripheral blood stem cells and umbilical cord blood stem cells;
• the first medium is a serum-free DMEM/F12 medium comprising TGF-b1, FGF-2, hydrocortisone, human serum albumin, L-glutamine, leucine aminopeptidase, an HEPES buffer and lipid;
• the first culture time is between 1 day and 10 days;
• the second culture time is between 7 days and 28 days;
• the optimized cell transplant has simultaneously and highly expressed CD200, Galectin-9 and VISTA genes;
• and
• the expression levels of the CD200, Galectin-9 and VISTA genes of the optimized cell transplant are at least two times higher than those of the mesenchymal stem cells.