Real-time sampling system

A system comprising:

• an elongated instrument;
• a control device movably couplable with the elongated instrument and configured to extend and retract the elongated instrument;
• a coupling configured to removably secure the control device with an insertion device, the insertion device being configured to convey the elongated instrument to a target location, the insertion device including a port to receive the elongated instrument therethrough;
• a bushing including a cylindrical tube that extends from the coupling and configured to be inserted within an opening in the port of the insertion device, the elongated instrument being movably extendable through the bushing;
• a first sealing member affixed circumferentially around an outer cylindrical surface of the cylindrical tube forming the bushing adjacent a distal end of the bushing and including an angled sealing surface forming a distal end of the first sealing member to seal the outer cylindrical surface of the bushing against an inner surface of the opening in the port;
• and
• a second sealing member a portion of which forms a proximalmost end surface of the bushing and configured to slidably receive an outer surface of the elongated instrument to form a movable seal between the outer surface of the elongated instrument and an inner surface of the proximal end of the bushing.

A method comprising:

• presenting a coupling adjacent to an opening in a port of an insertion device, the coupling supporting an elongated instrument to be conveyed to a target location by the insertion device;
• inserting a bushing including a cylindrical tube into the opening in the insertion device through which the elongated instrument is extendable;
• sealing an outer surface of the bushing against the opening to prevent fluid from passing between an inner surface of the opening and the outer surface of the bushing, wherein the sealing the outer surface includes engaging an angled sealing surface forming a distal end of a first sealing member against an inner surface of the opening in the port, wherein the first sealing member is affixed circumferentially around and in physical contact with an outer cylindrical surface of the cylindrical tube forming the bushing adjacent a distal end of the bushing;
• and
• movably sealing using a second sealing member including a portion forming a proximalmost end surface of the bushing, the second sealing member slidably disposed on an outer surface of the elongated instrument to form a movable seal between the outer surface of the elongated instrument and an inner surface of the proximal end of the bushing to prevent the fluid from passing between the outer surface of the elongated instrument and the coupling, such that fluid is sealably prevented from passing into the coupling around the outer surface of the bushing and around the outer surface of the elongated instrument.