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US 12,660,788 B2Granted
Tobamovirus-resistant tomato plant, method for producing Tobamovirus-resistant tomato plant, method for imparting Tobamovirus resistance in tomato plant, method for screening for Tobamovirus-resistant tomato plant, and method for detecting Tobamovirus resistance in tomato plant
AssigneesNational Agriculture and Food Research Organization, Takii & Company Limited
InventorsMasayuki Ishikawa, Kazuhiro Ishibashi, Akihito Kano
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Abstract
The present invention provides a tomato plant which is resistant to ToBRFV. A Tobamovirus resistant tomato plant of the present invention is with loss of function for a SlTOM1a gene, a SlTOM1c gene, and a SlTOM1d gene.
Claims12
A method for producing a Tobamovirus resistant tomato plant, comprising the following step (a):
- (a) crossing a Tobamovirus resistant tomato plant with another tomato plant,
- wherein the Tobamovirus resistant tomato plant comprises a loss-of-function for a Solanum lycopersicum TOM1a (SITOM1a) gene, a Solanum lycopersicum TOM1c (SITOM1c) gene, and a Solanum lycopersicum TOM1d (SITOM1d) gene, wherein
- the SITOM1a gene is a Tobamovirus resistance control gene comprising the following polynucleotide (NA):
- (NA) any of the following polynucleotides (NA1) to (NA3) and (NA5) to (NA7):
- (NA1) a polynucleotide consisting of a base sequence of SEQ ID NO:
- 1 or 2;
- (NA2) a polynucleotide consisting of a base sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 26 bases in a base sequence of the polynucleotide (NA1), and encoding a protein having Tobamovirus resistance control activity;
- (NA3) a polynucleotide consisting of a base sequence having at least 98% sequence identity to the base sequence of the polynucleotide (NA1), and encoding a protein having Tobamovirus resistance control activity;
- (NA5) a polynucleotide encoding a protein consisting of an amino acid sequence of SEQ ID NO:
- 3;
- (NA6) a polynucleotide encoding a protein consisting of an amino acid sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 6 amino acids in the amino acid sequence of SEQ ID NO:
- 3, and having Tobamovirus resistance control activity;
- and
- (NA7) a polynucleotide encoding a protein consisting of an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:
- 3, and having Tobamovirus resistance control activity,
- the SITOM1c gene is a Tobamovirus resistance control gene comprising the following polynucleotide (NC):
- (NC) any of the following polynucleotides (NC1) to (NC3) and (NC5) to (NC7):
- (NC1) a polynucleotide consisting of a base sequence of SEQ ID NO:7 or 8;
- (NC2) a polynucleotide consisting of a base sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 26 bases in a base sequence of the polynucleotide (NC1), and encoding a protein having Tobamovirus resistance control activity;
- (NC3) a polynucleotide consisting of a base sequence having at least 98% sequence identity to the base sequence of the polynucleotide (NC1), and encoding a protein having Tobamovirus resistance control activity;
- (NC5) a polynucleotide encoding a protein consisting of an amino acid sequence of SEQ ID NO:
- 9;
- (NC6) a polynucleotide encoding a protein consisting of an amino acid sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 6 amino acids in the amino acid sequence of SEQ ID NO:
- 9, and having Tobamovirus resistance control activity;
- and
- (NC7) a polynucleotide encoding a protein consisting of an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:
- 9, and having Tobamovirus resistance control activity,
- the SITOM1d gene is a Tobamovirus resistance control gene comprising the following polynucleotide (ND):
- (ND) any of the following polynucleotides (ND1) to (ND3) and (ND5) to (ND7):
- (ND1) a polynucleotide consisting of a base sequence of SEQ ID NO:
- 10;
- (ND2) a polynucleotide consisting of a base sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 26 bases in a base sequence of the polynucleotide (ND1), and encoding a protein having Tobamovirus resistance control activity;
- (ND3) a polynucleotide consisting of a base sequence having at least 98% sequence identity to the base sequence of the polynucleotide (ND1), and encoding a protein having Tobamovirus resistance control activity;
- (ND5) a polynucleotide encoding a protein consisting of an amino acid sequence of SEQ ID NO:
- 11;
- (ND6) a polynucleotide consisting of an amino acid sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 6 amino acids in the amino acid sequence of SEQ ID NO:
- 11, and encoding a protein having Tobamovirus resistance control activity;
- and
- (ND7) a polynucleotide consisting of an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:
- 11, and encoding a protein having Tobamovirus resistance control activity,
- the Tobamovirus is Tomato mosaic virus (ToMV), or Tomato brown rugose fruit virus (ToBRFV), and
- the loss of function for the SITOM1a gene, the SITOM1c gene, and the SITOM1d gene is caused by introducing deletion, substitution, insertion, and/or addition of nucleotides into said genes itself.
39 more elements
A method for screening a Tobamovirus resistant tomato plant, comprising the step of:
- selecting for a tomato plant to be examined with loss of function for a SITOM1a gene, a SITOM1c gene, and a SITOM1d gene from one or more tomato plants to be examined as a Tobamovirus resistant tomato plant, wherein
- the SITOM1a gene is a Tobamovirus resistance control gene comprising the following polynucleotide (NA):
- (NA) any of the following polynucleotides (NA1) to (NA3) and (NA5) to (NA7):
- (NA1) a polynucleotide consisting of a base sequence of SEQ ID NO:
- 1 or 2;
- (NA2) a polynucleotide consisting of a base sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 26 bases in a base sequence of the polynucleotide (NA1), and encoding a protein having Tobamovirus resistance control activity;
- (NA3) a polynucleotide consisting of a base sequence having at least 98% sequence identity to the base sequence of the polynucleotide (NA1), and encoding a protein having Tobamovirus resistance control activity;
- (NA5) a polynucleotide encoding a protein consisting of an amino acid sequence of SEQ ID NO:
- 3;
- (NA6) a polynucleotide encoding a protein consisting of an amino acid sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 6 amino acids in the amino acid sequence of SEQ ID NO:
- 3, and having Tobamovirus resistance control activity;
- and
- (NA7) a polynucleotide encoding a protein consisting of an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:
- 3, and having Tobamovirus resistance control activity,
- the SITOM1c gene is a Tobamovirus resistance control gene comprising the following polynucleotide (NC):
- (NC) any of the following polynucleotides (NC1) to (NC3) and (NC5) to (NC7):
- (NC1) a polynucleotide consisting of a base sequence of SEQ ID NO:7 or 8;
- (NC2) a polynucleotide consisting of a base sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 26 bases in a base sequence of the polynucleotide (NC1), and encoding a protein having Tobamovirus resistance control activity;
- (NC3) a polynucleotide consisting of a base sequence having at least 98% sequence identity to the base sequence of the polynucleotide (NC1), and encoding a protein having Tobamovirus resistance control activity;
- (NC5) a polynucleotide encoding a protein consisting of an amino acid sequence of SEQ ID NO:
- 9;
- (NC6) a polynucleotide encoding a protein consisting of an amino acid sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 6 amino acids in the amino acid sequence of SEQ ID NO:
- 9, and having Tobamovirus resistance control activity;
- and
- (NC7) a polynucleotide encoding a protein consisting of an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:
- 9, and having Tobamovirus resistance control activity, and
- the SITOM1d gene is a Tobamovirus resistance control gene comprising the following polynucleotide (ND):
- (ND) any of the following polynucleotides (ND1) to (ND3) and (ND5) to (ND7):
- (ND1) a polynucleotide consisting of a base sequence of SEQ ID NO:
- 10;
- (ND2) a polynucleotide consisting of a base sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 26 bases in a base sequence of the polynucleotide (ND1), and encoding a protein having Tobamovirus resistance control activity;
- (ND3) a polynucleotide consisting of a base sequence having at least 98% sequence identity to the base sequence of the polynucleotide (ND1), and encoding a protein having Tobamovirus resistance control activity;
- (ND5) a polynucleotide encoding a protein consisting of an amino acid sequence of SEQ ID NO:
- 11;
- (ND6) a polynucleotide consisting of an amino acid sequence obtained by deletion, substitution, insertion, and/or addition of 1 to 6 amino acids in the amino acid sequence of SEQ ID NO:
- 11, and encoding a protein having Tobamovirus resistance control activity;
- and
- (ND7) a polynucleotide consisting of an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:
- 11, and encoding a protein having Tobamovirus resistance control activity,
- the Tobamovirus is Tomato mosaic virus (ToMV), or Tomato brown rugose fruit virus (ToBRFV), and
- the loss of function for the SITOM1a gene, the SITOM1c gene, and the SITOM1d gene is caused by introducing deletion, substitution, insertion, and/or addition of nucleotides into said genes itself.
38 more elements
Dependencies are inferred from the claim text.