Two phase indicator displacement assay

Abstract

The present disclosure provides for a method of detecting the displacement of a specific analyte, including providing an indicator in an organic phase to form a first composition; providing an aqueous phase with a test analyte to form a second composition; adding at least one organic solute to the organic phase; putting the first composition and second composition in contact with each other, thereby forming a lamellar phase and an interface between the first composition and second composition; and detecting the presence of the indicator in the organic phase, wherein the presence of the indicator in the organic phase indicates the presence of the specific analyte. The present disclosure also provides for a method of developing a high throughput assay for contemporaneous detection of multiple analytes. Further, the present disclosure provides for a biosensor for detection of a specific analyte.

Dependencies are inferred from the claim text.