Process for the preparation of lipidated proteinaceous structures

A process for the preparation of a conjugate, suitable to be inserted into hydrophobic environments selected from the group consisting of hydrophobic polymeric surfaces, lipid based drug delivery systems and membranes of living cells, wherein the conjugate comprises a biological molecule, an enzymatic tag, a hydrophilic spacer, a linker and a lipophilic moiety, wherein such process comprises enzymatic coupling of a component comprising the enzymatic tag, the hydrophilic spacer, the linker and the lipophilic moiety with the biological molecule in an aqueous medium and wherein such process comprises the following steps:

• a) preparing an aqueous dispersion of a component which has the formula (II):
• wherein
• m is any integer number from 15 to 60;
• n is any integer number from 3 to 27;
• p is any integer number from 0 to 9;
• b) adding a transpeptidase and the biological molecule which is a polypeptide selected from an antigen, a cell adhesion protein, a peptide hormone, a cytokine, or a receptor related to any of these molecules, an enzyme, or a natural or artificial antibody or fragment thereof;
• c) incubating the mixture obtained in step (b) to produce the conjugate with the biological molecule;
• d) purifying the conjugate obtained in step (c).