Standardization of lateral flow assay strip test results

A method, comprising:

• receiving a lateral flow assay (“LFA”) strip of a user to obtain a result value for the user from the LFA strip;
• reading the lateral flow assay (“LFA”) strip, using an imaging device, to generate an LFA strip image including a test line comprising a plurality of columns, the reading performed during a testing process to determine the result value for the user from the LFA strip;
• determining, using a processor, during the testing process, a standardized test result based on an intensity value of a portion of the LFA strip image using an equivalence curve stored in a memory, wherein the equivalence curve associates a plurality of intensity values with a plurality of equivalent cycle time (“eCt”) values, wherein using the equivalence curve comprises matching the intensity value of the portion of the LFA strip image with one of the plurality of intensity values of the equivalence curve to convert the matched intensity value to an eCt result value associated with the matched one of the plurality of intensity values;
• and
• providing, using the processor, the standardized test result.

A method comprising:

• building an equivalence curve for a lateral flow assay (“LFA”) strip, comprising:
• receiving, by a processor, test information for the LFA strip,
• receiving, by the processor, an image of the LFA strip;
• receiving, by the processor, a known concentration of a viral load placed onto the LFA strip;
• associating, by the processor, an intensity of a portion of the image of the LFA strip with the known concentration of the viral load placed onto the LFA strip and a standardized value;
• repeating one or more previous steps, by the processor, using varying concentrations of viral load, until the equivalence curve is created;
• and
• storing the equivalence curve in a memory;
• receiving a second lateral flow assay (“LFA”) strip of a user to obtain a result value for the user from the LFA strip;
• reading the second lateral flow assay (“LFA”) strip, using an imaging device, to generate an LFA strip image including a test line;
• determining, using the processor, a result for the LFA strip image based on an intensity value of a portion of the LFA strip image using the equivalence curve that associates an intensity value with a standardized test result to generate the result value for the user, wherein the equivalence curve that associates an intensity value with a standardized test result comprises an equivalence curve that associates a plurality of intensity values with a plurality of equivalent cycle time (“eCt values”), and wherein using the equivalence curve comprises matching the intensity value of the portion of the LFA strip image with one of the plurality of intensity values of the equivalence curve to generate an eCt value associated with the matched one of the plurality of intensity values.