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US 12,656,323 B2Granted
Fluidic configuration for a multidimensional liquid chromatography system
AssigneeWaters Technologies Corporation
InventorsMichael O. Fogwill, Sylvain Gilles Cormier, Thomas Swann, Francis Patrick Tracey
Life of this patent
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Abstract
Multidimensional liquid chromatography (MDLC) systems include valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Optionally, a single detector is used for both first and second dimensions. In addition, the systems can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.
Claims19
A multidimensional liquid chromatography system, comprising:
- a first switching valve;
- a second switching valve in communication with the first switching valve by a first fluidic path defined by tubing having a first end coupled to a first port of the first switching valve and a second end coupled to a first port of the second switching valve;
- a first dimension column and a second dimension column in communication with the first switching valve through a second port and a third port, respectively, of the first switching valve;
- a first detector in communication with the first switching valve through a second fluidic path defined by tubing having a first end coupled to a fourth port of the first switching valve and a second end coupled to a first port of the first detector and a third fluidic path defined by tubing having a first end coupled to a fifth port of the first switching valve and a second end coupled to a second port of the first detector, wherein the first switching valve uses the first detector for detecting an output of the first dimension column and an output of the second dimension column;
- and
- a second detector in communication with a second port of the second switching valve, the second switching valve enabling an independent use of a first separation of the first dimension column and a second separation of the second dimension column.
3 more elements
A multidimensional liquid chromatography system, comprising:
- a first switching valve;
- a second switching valve in communication with the first switching valve by a first fluidic path defined by tubing having a first end coupled to a first port of the first switching valve and a second end coupled to a first port of the second switching valve;
- a first dimension column and a second dimension column in communication with the first switching valve through a second port and a third port, respectively, of the first switching valve;
- a first detector in communication with the first switching valve through a second fluidic path defined by tubing having a first end coupled to a fourth port of the first switching valve and a second end coupled to a first port of the first detector and a third fluidic path defined by tubing having a first end coupled to a fifth port of the first switching valve and a second end coupled to a second port of the first detector;
- and
- a second detector in communication with a second port of the second switching valve, wherein in a first state of the first and second switching valves, a mobile phase from the second dimension column flows through the first switching valve and then to the second switching valve so that the first detector is used to monitor a second dimension separation, and in a second state of the first and second switching valves, an eluent from the second dimension column flows through the first and second switching valves and to the second detector.
3 more elements
A multidimensional liquid chromatography system, comprising:
- a first switching valve;
- a second switching valve in communication with the first switching valve by a first fluidic path defined by tubing having a first end coupled to a first port of the first switching valve and a second end coupled to a first port of the second switching valve;
- a first dimension column and a second dimension column in communication with the first switching valve through a second port and a third port, respectively, of the first switching valve;
- a first detector in communication with the first switching valve through a second fluidic path defined by tubing having a first end coupled to a fourth port of the first switching valve and a second end coupled to a first port of the first detector and a third fluidic path defined by tubing having a first end coupled to a fifth port of the first switching valve and a second end coupled to a second port of the first detector, wherein in a first state the first and second switching valves are configured to connect an outlet of the second dimension column to the first detector;
- and
- a second detector in communication with a second port of the second switching valve, wherein in a second state the first and second switching valves are configured to connect the outlet of the second dimension column to the second detector.
3 more elements
Dependencies are inferred from the claim text.