Compositions and methods for detecting plasmodium species nucleic acid
AssigneesGen-Probe Incorporated, Grifols Diagnostic Solutions Inc.
InventorsDeanna Self, Jeffrey M. Linnen, Vanessa Bres
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Abstract
Disclosed are nucleic acid oligomers, including amplification oligomers, detection probes, and capture probes, for detection of Plasmodium species nucleic acid in a sample. Also disclosed are methods of specific nucleic acid amplification and detection, including amplification and detection of target nucleic acid in real time, using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
Claims24
A method for specifically detecting Plasmodium species nucleic acid in a sample, said method comprising:
- (1) contacting a sample, said sample suspected of containing Plasmodium species nucleic acid, with at least two oligomers for amplifying a target region of a Plasmodium species target nucleic acid, wherein the at least two amplification oligomers comprise:
- (a) at least one first amplification oligomer comprising a target-hybridizing sequence
- (i) that is from about 14 to about 20 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:
- 162, and comprises the sequence of SEQ ID NO:
- 163;
- or
- (ii) that is from about 14 to about 25 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:
- 166, and comprises the sequence of SEQ ID NO:
- 167 or SEQ ID NO:
- 168;
- and
- (b) at least one second amplification oligomer comprising a target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length, is contained in SEQ ID NO:
- 169 and comprises the sequence of SEQ ID NO:
- 171, SEQ ID NO:172, or SEQ ID NO:173;
- (2) performing an in vitro nucleic acid amplification reaction, wherein any Plasmodium target nucleic acid present in said sample is used as a template for generating an amplification product;
- and
- (3) contacting the in vitro nucleic acid amplification with at least one detection probe oligomer configured to specifically hybridize to the amplification product, wherein the at least one detection probe oligomer comprises a detectable label;
- and
- (4) detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Plasmodium species target nucleic acid in said sample.
A combination of oligomers for determining the presence or absence of Plasmodium species in a sample, said oligomer combination comprising:
- (1) at least two amplification oligomers for amplifying a target region of Plasmodium species target nucleic acid, wherein the at least two amplification oligomers comprise:
- (a) at least one first amplification oligomer comprising a target-hybridizing sequence
- (i) that is from about 14 to about 20 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:162, and comprises the sequence of SEQ ID NO:163;
- or
- (ii) that is from about 14 to about 25 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:166, and comprises the sequence of SEQ ID NO:
- 167 or SEQ ID NO:168;
- and
- (b) at least one second amplification oligomer comprising a target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length, is contained in SEQ ID NO:
- 169 and comprises the sequence of SEQ ID NO:
- 171, SEQ ID NO:
- 172, or SEQ ID NO:
- 173;
- and
- (2) at least one detection probe oligomer configured to specifically hybridize to an amplification product of the at least two amplification oligomers, wherein the at least one detection probe oligomer comprises a fluorescent label, a chemiluminescent label, or a fluorescent label and a non-fluorescent quencher.
Dependencies are inferred from the claim text.