DNA vaccines
AssigneeTouchlight IP Limited
InventorVanessa Hill
Life of this patent
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Abstract
An in vitro process for the production of closed linear deoxyribonucleic acid (DNA) comprises (a) contacting a DNA template comprising at least one protelomerase target sequence with at least one DNA polymerase in the presence of one or more primers under conditions promoting amplification of the template; and (b) contacting amplified DNA produced in (a) with at least one protelomerase under conditions promoting production of closed linear DNA. A kit provides components necessary in the process.
Claims30
A process for production of concatameric double-stranded deoxyribonucleic acid (DNA) suitable for use in in the production of linear double-stranded DNA covalently closed at both ends by hairpin loops, the process comprising:
- (a) amplifying by rolling circle amplification a linear double-stranded DNA template covalently closed at both ends by hairpin loops formed by the enzymatic activity of a protelomerase,
- wherein the process occurs in an in vitro environment.
A process for production of linear double-stranded deoxyribonucleic (DNA) acid covalently closed at both ends by hairpin loops, the process comprising:
- (a) amplifying by rolling circle amplification a linear double-stranded DNA template covalently closed at both ends by hairpin loops formed by the enzymatic activity of a protelomerase, to produce a product comprising multiple protelomerase target sequences;
- and
- (b) producing the linear double-stranded DNA covalently closed at both ends by hairpin loops by contacting the product comprising multiple protelomerase target sequences produced in (a) with at least one protelomerase under suitable conditions,
- wherein steps (a) and (b) occur in an in vitro environment.
Dependencies are inferred from the claim text.