Microfluidic nucleic acid detection kit and detection device

A microfluidic nucleic acid detection kit, comprising a kit body and a reagent card, wherein the kit body and the reagent card are interconnected, the kit body is configured to slide relative to the reagent card to control the microfluidic nucleic acid detection kit to work;

• the microfluidic nucleic acid detection kit further comprises a plurality of cavities and a plurality of flow channels, the plurality of flow channels communicates with the plurality of cavities;
• the plurality of cavities and the plurality of flow channels form a closed system;
• and the plurality of cavities at least comprises:
• a gas source cavity arranged in the kit body and configured for controlling a gas pressure of the closed system through a volume change;
• a liquid cavity arranged in the kit body and configured to inject a liquid reagent, wherein the liquid cavity communicates with the gas source cavity through a gas source flow channel;
• a nucleic acid amplification cavity arranged on the reagent card, wherein the nucleic acid amplification cavity communicates with the liquid cavity through a sample injection flow channel;
• a gas tail cavity arranged on the reagent card, wherein the gas tail cavity communicates with the nucleic acid amplification cavity through an exhaust gas flow channel;
• and
• a first sealing membrane arranged on the reagent card, wherein the first sealing membrane seals the sample injection flow channel, the exhaust gas flow channel, and the gas tail cavity.