Inhibitor of non-specific binding of nucleic acid, hybridization reagent and nucleic acid hybridization method
AssigneeToray Industries, Inc.
InventorTakashi Serizawa
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Abstract
A non-specific-binding inhibitor enables, in detection of a target nucleic acid by hybridization, effective inhibition of cross-hybridization between the target nucleic acid and complementary strands of sequences similar thereto, which non-specific-binding inhibitor has a stable quality among production lots, and a hybridization method for nucleic acid uses the inhibitor. The non-specific-binding inhibitor for nucleic acid includes a nucleic acid which has a base length of 2 to 11 bases and in which the content of a guanine base(s) and/or methylated guanine base(s) in the entire base sequence is not less than 70%.
Claims9
A method of hybridizing a target nucleic acid comprising:
- hybridizing the target nucleic acid with a nucleic acid for specific binding to the target nucleic acid in a hybridization solution,
- the hybridization solution comprising a non-specific-binding inhibitor for the nucleic acid, and wherein the non-specific-binding inhibitor is not immobilized on a substrate;
- the non-specific-binding inhibitor for the nucleic acid consisting essentially of a nucleic acid having a base length of five bases, six bases, seven bases, eight bases, nine bases, 10 bases, or 11 bases, and having from 5 to 10 consecutive guanine bases,
- wherein non-specific binding is decreased compared to when the non-specific-binding inhibitor is not added
- wherein the non-specific binding inhibitor is DNA, and
- wherein the target nucleic acid is human miRNA.
A method of detecting a target nucleic acid comprising:
- a hybridization step of hybridizing the target nucleic acid with a nucleic acid for specific binding to the target nucleic acid in a hybridization solution, wherein a non-specific-binding inhibitor for a nucleic acid is contained in the hybridization solution and not immobilized on a substrate;
- the non-specific-binding inhibitor consisting essentially of a nucleic acid having a base length of five bases, six bases, seven bases, eight bases, nine bases, 10 bases, or 11 bases, and having from 5 to 10 consecutive guanine bases
- wherein non-specific binding is decreased compared to when the non-specific binding inhibitor is not added;
- wherein the non-specific binding inhibitor is DNA,
- wherein the target nucleic acid is human miRNA, and
- further comprising a detection step of detecting a nucleic acid complex formed by the hybridization step.
Dependencies are inferred from the claim text.