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US 12,655,461 B2Granted
Biosensors for selectively identifying azide ions
AssigneeRutgers, The State University of New Jersey
InventorsChandra Kanth Bandi, Shishir Chundawat, Kyle S. Skalenko
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Abstract
The present disclosure provides, in one aspect, an azide-inducible system for controlled gene expression in E. coli. In another aspect, the present disclosure provides a high throughput screening method for the identification of new glycosynthases (e.g., mutant glycosyl hydrolases) with enhanced activity and unique substrate specificity.
Claims14
A construct comprising a cyn promoter and a cynR promoter, wherein the construct comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 2 and 8.
A plasmid vector comprising:
- a. the construct comprising a cyn promoter and a cynR promoter, wherein the construct comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 2 and 8;
- b. an ampicillin resistance gene;
- c. a gene encoding a protein of interest, wherein the gene is located downstream of the cyn promoter;
- and
- d. a cynR gene, wherein the cynR gene is located downstream of the cynR promoter, wherein the expression of the gene encoding the protein of interest is inducible by azide.
3 more elements
Dependencies are inferred from the claim text.