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US 12,655,454 B2Granted
Process for producing formate using oxygen-tolerant enzymes
AssigneeGwangju Institute of Science and Technology
InventorsIn Chan Kwon, Jae Hyun Cha
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Abstract
In a process for producing formate, a mixed enzyme by mixing hydrogenase (H2ase) with oxygen tolerance and formate dehydrogenase (FDH) with oxygen tolerance is prepared, and the mixed enzyme and a gas including H2, CO2 and NAD+ are mixed such that formate may be produced even in the presence of oxygen, and thereby utilizing hydrogen sources including oxygen, such as coke oven gas.
Claims6
A process for producing formate, the process comprising:
- preparing a mixed enzyme by mixing hydrogenase (H2ase) with oxygen tolerance and formate dehydrogenase (FDH) with oxygen tolerance;
- and
- mixing the mixed enzyme and a gas including H2, CO2 and Nicotinamide adenine dinucleotide phosphate (NAD+),
- wherein the hydrogenase (H2ase) with oxygen tolerance comprises the amino acid sequence of SEQ ID NO:
- 1,
- wherein the formate dehydrogenase (FDH) with oxygen tolerance comprises the amino acid sequence of SEQ ID NO:
- 6,
- wherein the gas including H2 contains 5% or less of O2.
5 more elements
Dependencies are inferred from the claim text.