Artificial expression constructs for selectively modulating gene expression in non-neuronal brain cells
AssigneeAllen Institute
InventorsJonathan Ting, Bosiljka Tasic, Boaz P. Levi, Tanya Daigle, Lucas T. Graybuck, Edward Sebastian Lein, John K. Mich, Adriana Estela Sedeño Cortés, Hongkui Zeng
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Abstract
Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in astrocytes, oligodendrocytes, microglia, pericytes, SMC, or endothelial cells.
Claims20
An artificial expression construct comprising (i) an enhancer having a sequence of SEQ ID NO:
- 13, SEQ ID NO:
- 371, or SEQ ID NO:
- 72, or a sequence having at least 95% sequence identity to the sequence of SEQ ID NO 13, SEQ ID NO:
- 371, or SEQ ID NO:
- 72;
- (ii) a promoter;
- and (iii) a heterologous encoding sequence.
A method for selectively expressing a heterologous encoding sequence within a population of cells in vivo or in vitro, the method comprising
- providing, in a sufficient dosage and for a sufficient time to a sample or subject comprising the population of cells, an administrable composition comprising an artificial expression construct, wherein the artificial expression construct comprises (i) an enhancer having a sequence of SEQ ID NO:
- 13, SEQ ID NO:
- 371, or SEQ ID NO:
- 72, or a sequence having at least 95% sequence to the sequence of SEQ ID NO 13, SEQ ID NO:
- 371, or SEQ ID NO:
- 72;
- (ii) a promoter;
- and (iii) the heterologous encoding sequence, thereby selectively expressing the heterologous encoding sequence within the population of cells.
Dependencies are inferred from the claim text.