PPO polypeptides tolerant to PPO-inhibiting herbicides and use thereof

A PPO polypeptide tolerant to a PPO inhibitor herbicide, which is characterized in that the polypeptide comprises the motif “LLLNYI” (SEQ ID NO:

• 50), wherein the leucine L at position 3 within the motif is substituted with other amino acid, and the tyrosine Y at position 5 is substituted with other amino acid;
• and wherein:
• as compared to the amino acid sequence of a wild-type rice PPO1, the amino acid sequence of the PPO polypeptide has mutations L423S/Y425I at positions corresponding to 423 and 425 of the amino acid sequence of wild-type rice PPO1 protein as set forth in SEQ ID NO:
• 1;
• or
• as compared to the amino acid sequence of a wild-type corn PPO1, the amino acid sequence of the PPO polypeptide has mutations L424S/Y426I at positions corresponding to 424 and 426 of the amino acid sequence of wild-type corn PPO1 protein as set forth in SEQ ID NO:
• 2;
• or
• as compared to the amino acid sequence of a wild-type oilseed rape PPO1, the amino acid sequence of the PPO polypeptide has mutations L424S/Y426I at positions corresponding to 424 and 426 of the amino acid sequence of wild-type oilseed rape PPO1 protein as set forth in SEQ ID NO:
• 3;
• or
• as compared to the amino acid sequence of a wild-type oilseed rape PPO1, the amino acid sequence of the PPO polypeptide has mutations L423S/Y425I at positions corresponding to 423 and 425 of the amino acid sequence of wild-type oilseed rape PPO1 protein as set forth in SEQ ID NO:
• 4;
• or
• as compared to the amino acid sequence of a wild-type peanut PPO1, the amino acid sequence of the PPO polypeptide has mutations L445S/Y447I at positions corresponding to 445 and 447 of the amino acid sequence of wild-type peanut PPO1 protein as set forth in SEQ ID NO:
• 5;
• or
• as compared to the amino acid sequence of a wild-type peanut PPO1, the amino acid sequence of the PPO polypeptide has mutations L439S/Y441I at positions corresponding to 439 and 441 of the amino acid sequence of wild-type peanut PPO1 protein as set forth in SEQ ID NO:
• 6;
• or
• as compared to the amino acid sequence of a wild-type soybean PPO1, the amino acid sequence of the PPO polypeptide has mutations L430S/Y432I at positions corresponding to 430 and 432 of the amino acid sequence of wild-type soybean PPO1 protein as set forth in SEQ ID NO:
• 7;
• or
• as compared to the amino acid sequence of a wild-type sorghum PPO1, the amino acid sequence of the PPO polypeptide has mutations L423S/Y425I at positions corresponding to 423 and 425 of the amino acid sequence of wild-type sorghum PPO1 protein as set forth in SEQ ID NO:
• 8;
• or
• as compared to the amino acid sequence of a wild-type wheat PPO1, the amino acid sequence of the PPO polypeptide has mutations L418S/Y420I at positions corresponding to 418 and 420 of the amino acid sequence of wild-type wheat PPO1 protein as set forth in SEQ ID NO:
• 9, 10 or 11;
• or
• as compared to the amino acid sequence of a wild-type tomato PPO1, the amino acid sequence of the PPO polypeptide has mutations s L445S/Y447I at positions corresponding to 445 and 447 of the amino acid sequence of wild-type tomato PPO1 protein as set forth in SEQ ID NO:
• 12;
• or
• as compared to the amino acid sequence of a wild-type potato PPO1, the amino acid sequence of the PPO polypeptide has mutations L444S/Y446I at positions corresponding to 444 and 446 of the amino acid sequence of wild-type potato PPO1 protein as set forth in SEQ ID NO:
• 13;
• or
• as compared to the amino acid sequence of a wild-type tobacco PPO1, the amino acid sequence of the PPO polypeptide has mutations L440S/Y442I at positions corresponding to 440 and 442 of the amino acid sequence of wild-type tobacco PPO1 protein as set forth in SEQ ID NO:
• 14;
• or
• as compared to the amino acid sequence of a wild-type Arabidopsis thaliana PPO1, the amino acid sequence of the PPO polypeptide has mutations L424S/Y426I at positions corresponding to 424 and 426 of the amino acid sequence of wild-type Arabidopsis thaliana PPO1 protein as set forth in SEQ ID NO:
• 15;
• or
• as compared to the amino acid sequence of a wild-type upland cotton PPO1, the amino acid sequence of the PPO polypeptide has mutations L426S/Y428I at positions corresponding to 426 and 428 of the amino acid sequence of wild-type upland cotton PPO1 protein as set forth in SEQ ID NO:
• 16;
• or
• as compared to the amino acid sequence of a wild-type radish PPO1, the amino acid sequence of the PPO polypeptide has mutations L425S/Y427I at positions corresponding to 425 and 427 of the amino acid sequence of wild-type radish PPO1 protein as set forth in SEQ ID NO:
• 17;
• or
• as compared to the amino acid sequence of a wild-type foxtail millet PPO1, the amino acid sequence of the PPO polypeptide has mutations L422S/Y424I at positions corresponding to 422 and 424 of the amino acid sequence of wild-type foxtail millet PPO1 protein as set forth in SEQ ID NO:
• 18;
• or
• as compared to the amino acid sequence of a wild-type cabbage PPO1, the amino acid sequence of the PPO polypeptide has mutations L424S/Y426I at positions corresponding to 424 and 426 of the amino acid sequence of wild-type cabbage PPO1 protein as set forth in SEQ ID NO:
• 19.