US 12,655,440 B2Granted

Genetically modified plants that exhibit an increase in seed yield comprising a first biotin attachment domain-containing (badc) gene homozygous for a wild-type allele, a second badc gene homozygous for a mutant allele, and homologs of the first and second badc genes

AssigneeNuseed Nutritional US Inc.

InventorsMeghna Malik, Jihong Tang, Yuanyuan Ji, Venkatesh Bollina, Lifeng Chen, Marie Mykytyshyn, Kristi D. Snell

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Abstract

A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant is provided. The genetically modified plant includes: (a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome and being homozygous for a wild-type allele: (b) a second badc gene, occurring in its natural position within the genome and being homozygous for a mutant allele; (c) one or more homologs of the first badc gene, each occurring in its natural position within the genome and being homozygous for a wild-type allele; and (d) one or more homologs of the second badc gene, each occurring in its natural position within the genome, and at least one being homozygous for a wild-type allele. The mutant allele does not encode a functional BADC protein. The increase in seed yield is at least 4%.

Claims11

Dependent claims

A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant from which the genetically modified plant was derived, the genetically modified plant comprising:

(a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;
(b) a second badc gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a mutant allele;
(c) one or more homologs of the first badc gene, each of the one or more homologs of the first badc gene occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;
and
(d) one or more homologs of the second badc gene, each of the one or more homologs of the second badc gene occurring in its natural position within the genome of the genetically modified plant, and at least one of the homologs of the second badc gene being homozygous for a wild-type allele, wherein:
(i) the wild-type alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene are identical to respective alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene from the progenitor plant;
(ii) the mutant allele of the second badc gene does not encode a functional BADC protein and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second badc gene from the progenitor plant;
(iii) the increase in seed yield is at least 4%;
(iv) the genetically modified plant is Brassica napus;
and
(v) the first badc gene or the one or more homologs of the first badc gene comprise Brassica napus badc1-1 of SEQ ID NO:
62 or badc1-2 of SEQ ID NO:
63.

10 more elements

A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant from which the genetically modified plant was derived, the genetically modified plant comprising:

(a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;
(b) a second badc gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a mutant allele;
(c) one or more homologs of the first badc gene, each of the one or more homologs of the first badc gene occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;
and
(d) one or more homologs of the second badc gene, each of the one or more homologs of the second badc gene occurring in its natural position within the genome of the genetically modified plant, and at least one of the homologs of the second badc gene being homozygous for a wild-type allele, wherein:
(i) the wild-type alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene are identical to respective alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene from the progenitor plant;
(ii) the mutant allele of the second badc gene does not encode a functional BADC protein and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second badc gene from the progenitor plant;
(iii) the increase in seed yield is at least 4%;
(iv) the genetically modified plant is Brassica napus;
and
(v) the second badc gene or the one or more homologs of the second badc gene comprises one or more of Brassica napus badc3-1 of SEQ ID NO:
64, badc3-2 of SEQ ID NO:
65, badc3-3 of SEQ ID NO:
66, or badc3-4 of SEQ ID NO:
67.

12 more elements

A genetically modified plant that exhibits an increase in seed yield relative to a progenitor plant from which the genetically modified plant was derived, the genetically modified plant comprising:

(a) a first biotin attachment domain-containing (badc) gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;
(b) a second badc gene, occurring in its natural position within the genome of the genetically modified plant and being homozygous for a mutant allele;
(c) one or more homologs of the first badc gene, each of the one or more homologs of the first badc gene occurring in its natural position within the genome of the genetically modified plant and being homozygous for a wild-type allele;
and
(d) one or more homologs of the second badc gene, each of the one or more homologs of the second badc gene occurring in its natural position within the genome of the genetically modified plant, and at least one of the homologs of the second badc gene being homozygous for a wild-type allele, wherein:
(i) the wild-type alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene are identical to respective alleles of the first badc gene, each of the one or more homologs of the first badc gene, and the at least one of the homologs of the second badc gene from the progenitor plant;
(ii) the mutant allele of the second badc gene does not encode a functional BADC protein and includes one or more additions, deletions, or substitutions of one or more nucleotides relative to an allele of the second badc gene from the progenitor plant;
(iii) the increase in seed yield is at least 4%;
(iv) the genetically modified plant is Brassica napus;
(v) the first badc gene and the one or more homologs of the first badc gene, each being homozygous for a wild-type allele, are Brassica napus badc1-1 of SEQ ID NO:
62and badc1-2 of SEQ ID NO:
63;
(vi) the second badc gene, being homozygous for a mutant allele, is Brassica napus badc3-2 of SEQ ID NO:
65;
(vii) the at least one homolog of the second badc gene, being homozygous for a wild-type allele, are Brassica napus badc3-1 of SEQ ID NO:
64 and badc3-4 of SEQ ID NO:
67;
and
(viii) a remaining homolog of the second badc gene, being homozygous for a mutant allele, is Brassica napus badc3-3 of SEQ ID NO:
66.

17 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/005,302Filed 12 Aug 2021 · us provisional application
Priority: 12 Aug 2020
Published: US 2023/0257759 A117 Aug 2023
Granted: 16 Jun 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 15/8247
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified lipid metabolism, e.g. seed…
A01H 5/10
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: seeds
A01H 6/20
Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy: brassicaceae, e.g. canola, broccoli or rucola
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants