Plant transactivation interaction motifs and uses thereof
AssigneeCorteva Agriscience LLC
InventorsJoseph F. Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Abstract
This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.
Claims2
A synthetic transcriptional activator fusion protein comprising:
- a single DNA-binding peptide;
- and
- a heterologous transactivation domain comprising the interaction motif peptide of SEQ ID NO:22, wherein the transactivation domain comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:
- 109 or SEQ ID NO:110.
A synthetic transcriptional activator fusion protein comprising:
- a single DNA-binding peptide;
- and
- a heterologous transactivation domain comprising the interaction motif peptide of SEQ ID NO:22, wherein the transactivation domain comprises SEQ ID NO:
- 109 or SEQ ID NO:110.
Dependencies are inferred from the claim text.