Back to results
US 12,655,438 B2Granted
Augmented sgRNAs and methods for their use to enhance somatic and germline plant genome engineering
AssigneeRegents of the University of Minnesota
InventorsDaniel F. Voytas, Evan Ellison
Life of this patent
Estimating the term…
Abstract
Methods and materials for increasing somatic and germline genome editing are provided herein. For example, provided herein are methods and materials for using augmented sgRNAs to increase somatic and germline genome editing.
Claims17
A method for generating a plant comprising a specific genomic DNA sequence modification, the method comprising:
- delivering an augmented sgRNA to a transgenic plant that expresses an RNA guided gene editing reagent, wherein the augmented sgRNA comprises (i) a sequence targeted to the specific genomic DNA sequence and (ii) a mobile RNA sequence;
- and
- recovering, from the plant to which the augmented sgRNA comprising the mobile RNA sequence was delivered, tissue with a genetic modification induced at the specific genomic DNA sequence by the RNA-guided gene editing reagent, wherein the tissue is capable of transmitting the genetic modification to a next generation plant.
A method for generating a plant comprising a specific genomic DNA sequence modification, the method comprising:
- delivering (a) an augmented sgRNA and (b) a sequence encoding an RNA guided gene editing reagent to a plant, wherein the augmented sgRNA comprises (i) a sequence targeted to the specific genomic DNA sequence and (ii) a first mobile RNA sequence, and wherein the sequence encoding the RNA guided gene editing reagent comprises a second mobile RNA sequence;
- and
- recovering, from the plant to which the augmented sgRNA comprising the mobile RNA sequence was delivered, tissue with a genetic modification induced at the specific genomic DNA sequence by the RNA-guided gene editing reagent, wherein the tissue is capable of transmitting the genetic modification to a next generation plant.
Dependencies are inferred from the claim text.