Compositions and methods for modulating the gastrointestinal tract using bile salt hydrolases
AssigneeNorth Carolina State University
InventorsRodolphe Barrangou, Casey Theriot, Sarah O'Flaherty, Matthew Foley
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions and methods related to modulating the gastrointestinal tract. In particular, the present disclosure provides a novel therapeutic strategy for selective modulation of the gut microbiota bile acid pool using bile acid hydrolases (BSHs) for the prevention and treatment of diseases such as obesity, diabetes, Inflammatory bowel disease (IBD), liver and colon cancer, and Clostridioides difficile infections, among others.
Claims14
An engineered bacterial cell comprising a heterologous gene encoding a functional bile salt hydrolase polypeptide from Lactobacillus gasseri, wherein the heterologous gene encoding the bile salt hydrolase polypeptide comprises at least one mutation that produces at least one amino acid substitution in an alanine-methionine-isoleucine (AMI) motif at amino acid positions 259-261 of SEQ ID NO:
- 41, wherein the at least one amino acid substitution decreases bile acid substrate specificity.
Dependencies are inferred from the claim text.