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US 12,655,398 B2Granted
Multipartite luciferase
AssigneePromega Corporation
InventorsMary Hall, Lance P. Encell, Michael Killoran, Keith Wood, Thomas Smith, Virginia Kincaid, Thomas Kirkland, Melanie Dart
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Abstract
Provided herein are compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular, a bioluminescent complex is formed upon the interaction of two or more peptide tags (e.g., separately or fused as a dipeptide or tripeptide) and a polypeptide component.
Claims8
A composition comprising:
- (a) a peptide comprising an amino acid sequence having 100% sequence identity with SEQ ID NO:
- 268, wherein the amino acid sequence is compared to the full length of SEQ ID NO:
- 268, wherein a bioluminescent signal produced in the presence of a coelenterazine substrate is capable of substantial increase when the peptide contacts a second peptide consisting of SEQ ID NO:
- 25 and a polypeptide complement consisting of SEQ ID NO:
- 17 when compared to a bioluminescent signal produced by (1) the peptide and the coelenterazine substrate alone or (2) the peptide, the coelenterazine substrate, and only one of the second peptide or the polypeptide complement;
- (b) a nucleic acid comprising a sequence coding for the peptide of (a);
- (c) a fusion protein comprising the peptide of (a) and additional amino acid sequence;
- or
- (d) a nucleic acid comprising a sequence coding for the fusion protein of (c).
6 more elements
Dependencies are inferred from the claim text.