Construction and application of recombinant H5N8 subtype avian influenza virus carrying mapple fluorescence reporter gene

Abstract

Disclosed is an NS protein, comprising an amino acid sequence from positions 1 to 217 of SEQ ID NO: 2, an amino acid sequence of an mApple fluorescence reporter gene and an amino acid sequence from positions 480 to 600 of SEQ ID NO: 2 linked in order. A recombinant H5N8 subtype avian influenza virus carrying an mApple fluorescence reporter gene is further disclosed, a genomic RNA of which is composed of 7 RNA fragments (PB2, PB1, PA, HA, NP, NA, M) identical to those of the genomic RNA of a wild H5N8 subtype avian influenza virus, and an RNA fragment 8 encoding the NS protein. Compared with a wild H5N8 virus, the recombinant H5N8 subtype avian influenza virus has a weakened virulence, and can replicate in an SPF chicken lung and shows pathogenicity.

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