Ovarian cancer organoid culture
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsThomas F. Meyer, Mirjana Kessler
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Abstract
The present invention relates to a method for the production of a culture, e.g. an organoid culture of ovarian cancer or cancer precursor cells, particularly of high grade serous ovarian carcinoma cells. By means of this method, an organoid culture of ovarian cancer or cancer precursor cells and a biobank comprising a plurality of different organoid cultures of ovarian cancer or cancer precursor cells may be generated. Further, a culture medium suitable for the long-term culture of ovarian cancer or cancer precursor cells is provided. Furthermore, use of the organoid culture and the biobank for medical applications, e.g. in the field of diagnostics, and therapy and in the field of drug screening is described.
Claims9
A method for the production of an ovarian cancer or cancer precursor cell organoid culture, said method comprising:
- (a) isolating ovarian cancer or cancer precursor cells obtained from a human patient with ovarian carcinoma,
- (b) cultivating said ovarian cancer or cancer precursor cells in a cell culture medium, wherein:
- the cell culture medium comprises at least one compound which stimulates Bone Morphogenetic Protein (BMP) signaling and at least one compound selected from the group consisting of a serum-free neuronal supplement, nicotine amide, a ROCK inhibitor, an N-2 supplement and a TGF β inhibitor, and
- the cell culture medium:
- (i) is free of Noggin and free from exogenously added Wnt protein, and comprises EGF at a concentration of about 1-200 ng/ml and BMP2 at a concentration of about 1-50 ng/ml, or
- (ii) is free of Noggin and free from exogenously added Wnt protein, and comprises EGF at a concentration of about 1-200 ng/ml, FGF at a concentration of about 20-500 ng/ml, and Rspondin at a concentration of about 50-100 ng/ml, and
- (c) obtaining an organoid cell culture from the cultivation step (b), and
- (d) culturing said organoid cell culture.
Dependencies are inferred from the claim text.