Hair follicle primordia and method for producing same
AssigneesNational University Corporation Yokohama National University, Kanagawa Institute of Industrial Science and Technology
InventorsJunji Fukuda, Tatsuto Kageyama, Akihiro Shimizu, Rikuma Nakajima, Riki Anakama
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Abstract
Provided is a hair follicle germ capable of forming a hair shaft-like structure in vitro simply and within a short period of time. A method of producing a hair follicle germ includes: inoculating epithelial cells and mesenchymal cells; maintaining the epithelial cells and the mesenchymal cells in a culture solution in which (a) laminin and entactin, and/or (b) type IV collagen is dispersed; and co-culturing the epithelial cells and the mesenchymal cells in a culture solution to form a hair follicle germ.
Claims19
A method of producing a hair follicle germ, comprising:
- inoculating epithelial cells and mesenchymal cells;
- contacting the epithelial cells and the mesenchymal cells, before a hair follicle germ containing the epithelial cells and the mesenchymal cells is formed, with a first culture solution having fluidity at a temperature of 30° C. or more and 45° C. or less and comprising (a) laminin and entactin, and/or (b) type IV collagen;
- and
- co-culturing the epithelial cells and the mesenchymal cells in a second culture solution to form the hair follicle germ,
- wherein the contacting is performed before 28 hours have passed from a time point when the co-culturing is started, and
- wherein the epithelial cells and the mesenchymal cells are inoculated in a culture solution in which the (a) and/or the (b) is dispersed;
- or a culture solution in which the (a) and/or the (b) is not dispersed.
Dependencies are inferred from the claim text.