Back to results
US 12,655,385 B2Granted
Cell culture compositions with antioxidants and methods for polypeptide production
AssigneeGenentech, Inc.
InventorsNatarajan Vijayasankaran, Steven J. Meier, Sharat Varma, Yi Yang
Life of this patent
Estimating the term…
Abstract
Cell culture media comprising antioxidants are provided herein as are methods of using the media for cell culturing and polypeptide production from cells. Compositions comprising polypeptides, such as therapeutic polypeptides, produced by the methods herein are also provided.
Claims28
A method of making an anti-CD20 antibody composition comprising:
- (a) culturing Chinese Hamster Ovary (CHO) cells comprising a nucleic acid encoding the anti-CD20 antibody in a cell culture medium comprising one or more of components (i)-(v):
- (i) hypotaurine,
- (ii) s-carboxymethylcysteine,
- (iii) butylated hydroxyanisole,
- (iv) quercitrin hydrate, and
- (v) aminoguanidine;
- (b) producing the anti-CD20 antibody using the CHO cells;
- (c) purifying the anti-CD20 antibody from the cell culture medium;
- and
- (d) concentrating the anti-CD20 antibody of step (c) to make the anti-CD20 antibody composition.
7 more elements
A method of making a pharmaceutical formulation comprising combining:
- (1) a concentrated anti-CD20 antibody composition, and
- (2) a pharmaceutically acceptable carrier;
- wherein, the concentrated anti-CD20 antibody composition is made by:
- (a) culturing Chinese Hamster Ovary (CHO) cells comprising a nucleic acid encoding the anti-CD20 antibody in a cell culture medium comprising one or more of components (i)-(v):
- (i) hypotaurine,
- (ii) s-carboxymethylcysteine,
- (iii) butylated hydroxyanisole,
- (iv) quercitrin hydrate, and
- (v) aminoguanidine;
- (b) producing the anti-CD20 antibody using the CHO cells;
- (c) purifying the anti-CD20 antibody from the cell culture medium;
- and
- (d) concentrating the anti-CD20 antibody to make the concentrated anti-CD20 antibody composition.
10 more elements
Dependencies are inferred from the claim text.