Surface-modified cell culture substrates and methods of modifying cell culture substrates
AssigneeCorning Incorporated
InventorsTheresa Chang, Paula Jean Dolley-Sonneville, Paul Ernest Gagnon, Jr., Vinalia Tjong, Yue Zhou
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Abstract
A cell culture substrate is provided that includes a substrate lattice having an ordered array of fibers and pores disposed between the fibers. The ordered array of fibers includes a cell culture surface to support adherent or semi-adherent cells during cell culture. The cell culture substrate further includes a positive charge coating disposed on the cell culture surface to promote adhesion of cells to the cell culture surface.
Claims16
A cell culture substrate comprising:
- a substrate lattice comprising an ordered array of fibers and pores disposed between the fibers, the ordered array of fibers comprising a cell culture surface configured to support adherent or semi-adherent cells during cell culture;
- and
- a positive charge coating disposed on the cell culture surface, the positive charge coating being configured to promote adhesion of cells to the cell culture surface,
- wherein the positive charge coating is selected from a group consisting of:
- a plasma-deposited coating, a silane-based amine coating, and a photoactive polymer coating.
A packed-bed bioreactor system for culturing cells, the system comprising:
- a vessel comprising a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet;
- a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate comprising a porous substrate comprising a surface configured to culture cells thereon;
- and
- a positive charge coating disposed on the surface of the porous substrate, the positive charge coating being configured to promote adhesion of cells to the surface,
- wherein the positive charge coating is selected from a group consisting of:
- a plasma-deposited coating, a silane-based amine coating, and a photoactive polymer coating.
A method of making a cell culture substrate, the method comprising:
- providing a substrate lattice comprising an ordered array of pores disposed between connecting members of the lattice, the connecting members comprising a cell culture surface configured to support adherent or semi-adherent cells during cell culture;
- depositing a polymer coating on the cell culture surface of the substrate lattice, the polymer coating having a net positive charge,
- wherein the depositing of the polymer coating comprises treating the substrate lattice using plasma.
A method of making a cell culture substrate, the method comprising:
- providing a substrate lattice comprising an ordered array of pores disposed between connecting members of the lattice, the connecting members comprising a cell culture surface configured to support adherent or semi-adherent cells during cell culture;
- depositing a polymer coating on the cell culture surface of the substrate lattice, the polymer coating having a net positive charge,
- wherein the polymer coating comprises at least one of:
- a photoactive polymer coating, the photoactive polymer coating comprising at least one of an acrylamide, a methacrylamide, and an aminopropyl-methacrylamide, and
- a silane-based amine coating.
Dependencies are inferred from the claim text.