Automated cell culturing

An automated cell culture system comprising:

• a cell culture reactor comprising:
• a housing;
• a fluidic circuit for cell culture media, the fluidic circuit disposed in an interior of the housing, the fluidic circuit comprising:
• a culture vessel for culturing cells in the cell culture media,
• a reservoir for the cell culture media, the reservoir fluidically connected to the culture vessel, and
• a pump configured to pump the cell culture media in the fluidic circuit;
• sensors disposed in the interior of the housing and configured to detect parameters indicative of a phase of the cell culture, wherein the parameters include culture solution volume, pH value, dissolved oxygen, glucose concentration, and lactic acid concentration;
• a computing device comprising one or more processors and a memory for controlling an operation of the automated cell culture system, wherein the computing device is configured to:
• automatically determine a phase of the cell culture based on the detected parameters, wherein the phase of the cell culture is selected from an initial growth phase, a rapid growth phase, and a plateau, and
• automatically control a flow rate of cell culture media exchange within the cell culture reactor based on a comparison between at least the parameters indicative of the phase of the cell culture and a threshold, wherein the threshold depends on the phase of the cell culture, wherein the computing device is configured to control the flow rate of cell culture media exchange to be a first flow rate that maintains a first threshold volume of cell culture media when the cell culture is in the initial growth phase and a second flow rate that maintains a second threshold volume of cell culture media when the cell culture is in the rapid growth phase, wherein the first threshold volume during the initial growth phase is lower than the second threshold volume during the rapid growth phase, wherein the first flow rate during the initial growth phase is lower than the second flow rate during the rapid growth phase:
• a supply system comprising:
• a supply line, a first end of the supply line being connected to the fluidic circuit, and a second end of the supply line connectable to a source of cell culture media;
• a supply pump coupled to the supply line;
• and
• a temperature control system comprising:
• a control housing, an interior space of the control housing configured to house the source of cell culture media, and a temperature controller configured to cool or warm the interior space of the control housing,
• wherein the computing device is configured to:
• control operation of the supply pump based on (i) an amount of the cell culture media in the reservoir and (ii) a pH of the cell culture media in the fluidic circuit;
• and
• a gas source fluidically coupled to the interior of the housing, and a gas flow controller coupled to the gas source,
• wherein the computing device is configured to:
• control operation of the gas flow controller based on the parameters indicative of the phase of the cell culture to maintain a concentration of carbon dioxide and a concentration of oxygen within preset ranges, wherein the concentration of oxygen is maintained at 5%.