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US 12,655,368 B2Granted
Mannanase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsVivek Srivastava, Markus Klinger, Rakhi Saikia, Vijaya Shankar Nataraj, Sohel Dalal, Jens Erik Nielsen
Life of this patent
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Abstract
The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims20
A variant of a parent mannanase, which comprises
- (a) a first substitution at one or more positions corresponding to positions selected from the group consisting of:
- (i) 260 with Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Lys, Met, Phe, Pro, Ser, Thr, Tyr, or Val;
- (ii) 288 with Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- (iii) 294 with Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val;
- and
- (iv) 295 with Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Thr, Trp, Tyr, or Val;
- and
- (b) a second substitution at one or more other positions,
- wherein
- numbering is according to SEQ ID NO:
- 2,
- the variant comprises an amino acid sequence that has at least 80% and less than 100% sequence identity to the amino acid sequence of SEQ ID NO:
- 2, and
- the variant has mannanase activity.
11 more elements
A variant of a parent mannanase, which comprises
- (a) a first substitution at the position corresponding to positions selected from the group consisting of:
- (i) 260 with Leu;
- (ii) 288 with Ile;
- (iii) 294 with Ile;
- and
- (iv) 295 with Ala;
- and
- (b) a second substitution at one or more other positions,
- wherein
- numbering is according to SEQ ID NO:
- 2,
- the variant comprises an amino acid sequence that has at least 85% and less than 100% sequence identity to the amino acid sequence of SEQ ID NO:
- 2, and
- the variant has mannanase activity.
11 more elements
Dependencies are inferred from the claim text.