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US 12,655,180 B2Granted
Capsid variants and methods of using the same
AssigneeDyno Therapeutics, Inc.
InventorsIna Chen, Jeff Gerold, Jerrah Holth, Sylvain Lapan, Kathy S. Lin, Samuel Wolock
Life of this patent
Estimating the term…
Abstract
The disclosure is directed in part to variant capsid polypeptides that can be used to deliver payloads.
Claims24
A variant adeno-associated virus (AAV) capsid polypeptide having at least 95% sequence identity to the VP1 polypeptide of SEQ ID NO:2 or a VP2 or VP3 portion thereof and comprising:
- (a) a valine at a position corresponding to T593 as compared to SEQ ID NO:
- 1;
- (b) a leucine at a position corresponding to V596 as compared to SEQ ID NO:
- 1;
- and
- (c) a serine at a position corresponding to N598 as compared to SEQ ID NO:
- 1.
4 more elements
A recombinant AAV comprising:
- (a) an AAV capsid comprising AAV capsid polypeptides, each having at least 95% sequence identity to SEQ ID NO:2 and comprising:
- (i) a valine at a position corresponding to T593 as compared to SEQ ID NO:
- 1;
- (ii) a leucine at a position corresponding to V596 as compared to SEQ ID NO:
- 1;
- and
- (c) a serine at a position corresponding to N598 as compared to SEQ ID NO:
- 1;
- and
- (b) a minigene having AAV inverted terminal repeats and a transgene comprising a heterologous gene operably linked to regulatory sequences which direct expression of the heterologous gene in a host cell.
7 more elements
Dependencies are inferred from the claim text.