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US 12,655,168 B2Granted
Isolation of steviol glycosides
AssigneeDsm IP Assets B.V.
InventorsIgor Galaev, Ferdinand Antoine Spros
Life of this patent
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Abstract
The disclosure relates to the field of food ingredients, specifically to sweeteners, more specifically to steviol glycosides and improved isolation thereof.
Claims17
A process for the production of one or more steviol glycosides, the process comprising:
- culturing recombinant cells capable of producing the one or more steviol glycosides in a culture medium to provide a culture broth comprising the culture medium and the recombinant cells, wherein the one or more steviol glycosides are produced and precipitate out of the culture broth;
- and
- heating the culture broth comprising the recombinant cells to 45-65° C. for a period of at most 90 seconds such that the precipitated one or more steviol glycosides are at least partially re-solubilized in the culture broth.
A process for the recovery of one or more steviol glycosides from a culture broth wherein said culture broth comprises:
- recombinant cells capable of producing one or more steviol glycosides, a culture medium, one or more precipitated steviol glycosides, and optionally one or more solubilised steviol glycosides,
- wherein the process comprises heating the culture broth to 45-65° C. for a period of at most 90 seconds such that the precipitated one or more steviol glycosides are re-solubilized in the culture broth.
Dependencies are inferred from the claim text.