Point of need fluid transport device

A point of care apparatus comprising:

• a fluid container configured to receive a biofluid, the fluid container having an open end, and wherein the open end of the fluid container includes an engageable portion;
• an intermediate cap having a chamber formed between a first and second pierceable seal, the intermediate cap having an upwardly extending peripheral wall and a downwardly extending peripheral wall relative to the chamber, wherein the upwardly extending peripheral wall includes an engagement feature that couples the intermediate cap to the engageable portion of the fluid container;
• a cartridge having at least one opening in a top surface that is in fluid communication with at least one microfluidic channel that extends within the cartridge, the cartridge having a collar that extends from the top surface and defines a passageway that is in fluid communication with the at least one opening in the cartridge, the collar having an engageable portion, wherein the downwardly extending peripheral wall of the intermediate cap includes an engagement feature that couples the cartridge with the engageable portion of the collar;
• a first piercing insert configured between the first pierceable seal and the open end of the fluid container, the first piercing insert having a piercing feature and an opening extending through the first piercing insert, wherein engagement between the engagement feature of the upwardly extending peripheral wall of the intermediate cap and the engageable portion of the fluid container bias the piercing feature of the first piercing insert to pierce the first pierceable seal to place the fluid container in fluid communication with the chamber of the intermediate cap;
• and
• a second piercing insert configured between the second pierceable seal and the top surface of the cartridge, the second piercing insert having a piercing feature, wherein engagement between engagement feature of the downwardly extending peripheral wall of the intermediate cap and the engageable portion of the collar bias the piercing feature of the second piercing insert to pierce the second pierceable seal to place the at least one microfluidic channel in fluid communication with the chamber of the intermediate cap.