Microfluidic cellular device and methods of use thereof

A method for concentrating cells with a microfluidics device comprising a microfluidics chamber, an input mechanism, a first output mechanism, and a second output mechanism, the method comprising:

• introducing a first solution comprising cells into the microfluidics chamber via the input mechanism, wherein the microfluidics chamber is in fluid communication with the input mechanism;
• applying pressure to cause the solution comprising the cells to flow through the microfluidics chamber, wherein the microfluidics chamber comprises a plurality of rows of posts, wherein each row comprises a plurality of posts distributed along a line oriented in a diagonal manner with respect to the microfluidics chamber;
• wherein each post spans the height of the microfluidics chamber and is in contact with a ceiling and a floor of the microfluidics chamber, wherein each post has a length and a width and each post is shaped as a rectangle or an oval, and wherein the axis along the length of each post has a rotational angle (φ) defined in a counter clockwise or clockwise direction with respect to a side of the microfluidics chamber, wherein the rotational angle is between 1 and 89 degrees;
• and
• deflecting the cells to a side of the microfluidics chamber by the rows of posts to deplete cells from the solution exiting the first output mechanism and enrich cells in the solution exiting the second output mechanism.

A method for sorting activated T cells with a first microfluidics device comprising a first microfluidics chamber, a first input mechanism, a first output mechanism, and a second output mechanism, the method comprising:

• a first sorting comprising:
• introducing a first solution comprising activated T cells into the first microfluidics chamber via the first input mechanism, wherein the first microfluidics chamber is in fluid communication with the first input mechanism;
• applying pressure to cause the first solution to flow through the first microfluidics chamber, wherein the first microfluidics chamber comprises a plurality of rows of posts, wherein each row comprises a plurality of posts distributed along a line oriented in a diagonal manner with respect to the first microfluidics chamber, wherein a gap distance between adjacent posts is configured to retain activated T cells and wherein each post spans a height of the microfluidics chamber and is in contact with a ceiling and a floor of the microfluidics chamber, and wherein each post has a length and a width and each post is shaped as a rectangle or an oval, and wherein the axis along the length of each post has a rotational angle (φ) defined in a counter clockwise or clockwise direction with respect to a side of the microfluidics chamber, wherein the rotational angle is between 1 and 89 degrees;
• and
• deflecting the activated T cells to a side of the first microfluidics chamber by the plurality of rows of posts to deplete activated T cells from a first waste solution exiting the first output mechanism and to enrich activated T cells in a first collection solution exiting the second output mechanism.

A method for concentrating cells with a microfluidics device comprising a microfluidics chamber, an input mechanism, a first output mechanism, and a second output mechanism, the method comprising:

• introducing a first solution comprising cells into the microfluidics chamber via the input mechanism, wherein the chamber is in fluid communication with the input mechanism;
• applying pressure to cause the first solution comprising the cells to flow through the microfluidics chamber, wherein the microfluidics chamber comprises a plurality of rows of posts, wherein each row comprises a plurality of posts distributed along a line oriented in a diagonal manner with respect to the microfluidics chamber, wherein each post spans a height of the microfluidics chamber and is in contact with a ceiling and a floor of the microfluidics chamber, and wherein each post has a length and a width and each post is shaped as a rectangle or an oval, and wherein the axis along the length of each post has a rotational angle (φ) defined in a counter clockwise or clockwise direction with respect to a side of the microfluidics chamber, wherein the rotational angle is between 1 and 89 degrees;
• and
• deflecting the cells to a side of the microfluidics chamber by the plurality of rows of posts to deplete cells from the solution exiting the first output mechanism and enrich cells in the solution exiting the second output mechanism,
• wherein a flow rate of the first solution through the microfluidics device is greater than 10 mL/hr and a sample:
• sheath flow ratio is about 1:1 to about 4:1.