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US 12,653,926 B2Granted
Extracellular matrix-derived gels and related methods
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsStephen F. Badylak, Donald Freytes
Life of this patent
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Abstract
Provided are methods for preparing gelled, solubilized extracellular matrix (ECM) compositions useful as cell growth scaffolds. Also provided are compositions prepared according to the methods as well as uses for the compositions. In one embodiment a device, such as a prosthesis, is provided which comprises an inorganic matrix into which the gelled, solubilized ECM is dispersed to facilitate in-growth of cells into the ECM and thus adaptation and/or attachment of the device to a patient.
Claims20
A method of preparing an extracellular matrix-derived gel comprising:
- (i) providing an acidic extracellular matrix (ECM) digest solution comprising solubilized extracellular matrix digested with an acid protease, wherein the extracellular matrix has not been dialyzed, (ii) providing a neutralizing solution comprising a base and/or buffer capable of bringing the acidic ECM digest solution to physiological pH;
- (iii) mixing the acidic ECM digest solution with the neutralizing solution to produce a neutralized digest solution, and (iv) gelling the neutralized digest solution at a temperature greater than 25° C.
Dependencies are inferred from the claim text.