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US 12,653,897 B2Granted
Pegylated porcine interferon and methods of use thereof
AssigneesElanco US Inc., Ambrx, Inc.
InventorsPeter Connor Canning, Nickolas Knudsen, Lillian Skidmore
Life of this patent
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Abstract
Disclosed herein are porcine interferon alpha variants (pINF-α) comprising a synthetic amino acid at select locations in pINF-α and a one or two amino acid insertion in the N-terminus after removal of the signal peptide. The pINF-α variants can further be pegylated. Methods of making and administering these compounds to treat virus infections in pigs and formulations comprising the variants are also provided.
Claims16
A porcine interferon-α (pIFN-α) variant comprising:
- (SEQ ID NO:
- 1)
- XaXbCDLPQTHSLAHTRALRLLAQMRRISPFSCLDHRRDFGSPHEAFG
- GNQVQKAQAMALVHEMLQQTFQLFSTEGSAAAWNESLLHQFYTGLDQQ
- LRDLEACVMQEAGLEGTPLLEEDSIRAVRKYFHRLTLYLQEKSYSPCA
- WEIVRAEVMRSFSSSRNLQDRLRKKE,
- wherein residue E103, E107, L112, Y136, or Q102 (numbering with respect to SEQ ID NO:
- 4) is substituted with a synthetic amino acid;
- and wherein XaXb are variable positions selected from the group consisting of no amino acids, a single proline residue, a proline-serine dipeptide, a single serine residue, a serine-glycine dipeptide, and a histidine.
6 more elements
A porcine interferon-α (pIFN-α) variant consisting of:
- (SEQ ID NO:
- 14)
- XaXbCDLPQTHSLAHTRALRLLAQMRRISPFSCLDHRRDFGSPHEAFG
- GNQVQKAQAMALVHEMLQQTFQLFSTEGSAAAWNELLHQFYTGLDQQL
- RDLEACVMQEAGL-Xaa-GTPLLEEDSIRAVRKYFHRLTLYLQEKSYS
- PCAWEIVRAEVMRSFSSSRNLQDRLRKKE,
- wherein XaXb are variable positions selected from the group consisting of a single proline residue, a proline-serine dipeptide, a single serine residue, a serine-glycine dipeptide and a histidine;
- wherein a residue corresponding to E107 (numbering with respect to SEQ ID NO:
- 4) is substituted with a synthetic amino acid selected from para-acetyl-phenylalanine (pAF), acetylglucosaminyl-L-serine, and N-acetylglucosaminyl-L-threonine;
- and said synthetic amino acid is pegylated with a 30 kDa linear PEG.
7 more elements
Dependencies are inferred from the claim text.