T-cell immunotherapy specific for WT-1
AssigneeFred Hutchinson Cancer Center
InventorsThomas M. Schmitt, Aude G. Chapuis, Philip D. Greenberg
Life of this patent
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Abstract
The present disclosure provides binding proteins specific for human Wilms tumor protein 1 (WT-1) epitopes, as well as host cells that express the binding proteins. Also provided are polynucleotides that encode a binding protein and vectors that comprise a polynucleotide. Related methods and uses of the presently disclosed compositions are provided for treating diseases or disorders associated with WT-1 expression, such as various cancers.
Claims32
A host cell, comprising a heterologous polynucleotide that encodes a binding protein comprising a T cell receptor (TCR) α chain variable (Vα) domain and a TCR β-chain variable (Vβ) domain,
- wherein the encoded TCR Vα and Vβ domains comprise CDR1α, CDR2α, CDR3α, CDR1β, CDR2β, and CDR3β amino acid sequences of:
- (i) SEQ ID NOs.:
- 26-28 and 38-40, respectively;
- or
- (ii) SEQ ID NOs.:
- 23, 27, 28, and 38-40, respectively,
- and wherein the encoded binding protein is capable of binding to a RMFPNAPYL (SEQ ID NO.:
- 94):
- human leukocyte antigen (HLA) complex.
An isolated polynucleotide encoding a binding protein, wherein the encoded binding protein comprises a TCR Vα domain and a TCR Vβ domain and is capable of binding to a RMFPNAPYL (SEQ ID NO.:
- 94):
- HLA complex, wherein the HLA optionally comprises HLA-A*0201, and
- wherein the encoded TCR Vα and Vβ domains comprise CDR1α, CDR2α, CDR3α, CDR1β, CDR2β, and CDR3β amino acid sequences of:
- (i) SEQ ID NOs.:
- 26-28 and 38-40, respectively;
- or
- (ii) SEQ ID NOs.:
- 23, 27, 28, and 38-40, respectively.
A binding protein comprising a T cell receptor (TCR) α chain variable (Vα) domain and a TCR β chain variable (Vβ) domain, wherein the encoded TCR Vα and Vβ domains comprise CDR1α, CDR2α, CDR3α, CDR1β, CDR2β, and CDR3β amino acid sequences of:
- (i) SEQ ID NOs.:
- 26-28 and 38-40, respectively;
- or
- (ii) SEQ ID NOs.:
- 23, 27, 28, and 38-40, respectively.
Dependencies are inferred from the claim text.