Methods of preserving mesenchymal stem cells
AssigneesEmory University, Children's Healthcare of Atlanta, Inc.
InventorsJacques Galipeau, Raghavan Chinnadurai
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Abstract
This disclosure relates to methods of preserving mesenchymal stromal/stem cells (MSCs) for use in clinical applications. In certain embodiments, this disclosure relates to methods of preserving MSCs comprising mixing MSCs with interferon-gamma prior to cryopreserving, freezing, or cooling the MSCs to a temperature below zero degrees Celsius.
Claims8
A method of treating or preventing a disease or medical condition comprising administering or transplanting an effective amount of a composition comprising mesenchymal stem cells made by mixing mesenchymal stem cells with interferon gamma at a concentration of 20 ng/mL in culture medium for 48 hours providing mixed mesenchymal stem cells, and cooling the mixed mesenchymal stem cells to a temperature below zero degrees Celsius providing frozen interferon gamma exposed mesenchymal stem cells, wherein mixing is prior to cooling the mixed mesenchymal stem cells, and
- thawing the frozen interferon gamma exposed mesenchymal stem cells to a temperature above zero degrees Celsius providing thawed interferon gamma exposed mesenchymal stem cells that express indoleamine 2,3-dioxygenase (IDO) mRNA with greater than a 100-fold change in concentration when compared to cryopreserved mesenchymal stem cells that were not mixed with interferon gamma prior to freezing.
Dependencies are inferred from the claim text.