Methods for production of platelets from pluripotent stem cells and compositions thereof
AssigneeAstellas Institute for Regenerative Medicine
InventorsQiang Feng, Shi-Jiang Lu, Robert P. Lanza
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Abstract
Methods for production of platelets from pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are provided. These methods may be performed without forming embryoid bodies or clusters of pluripotent stem cells, and may be performed without the use of stromal inducer cells. Additionally, the yield and/or purity can be greater than has been reported for prior methods of producing platelets from pluripotent stem cells. Also provided are compositions and pharmaceutical preparations comprising platelets, preferably produced from pluripotent stem cells.
Claims18
A cryopreserved composition comprising platelets produced by a method comprising subjecting megakaryocytes in a feeder free culture conditions to shear forces to cause the formation in culture of pro-platelets that release platelets, at least 60% of which are positive for CD41a expression, wherein:
- the platelets have a shelf-life of at least 8 to 15 days when stored at 22-24° C. and maintain viability for use in a human patient.
Dependencies are inferred from the claim text.