Protein payload release

A membrane-cleavable chimeric protein, oriented from N-terminal to C-terminal, having the formula:

• S-C-MT or MT-C-S
• wherein
• S comprises a secretable effector molecule,
• C comprises a protease cleavage site, and
• MT comprises a cell-membrane tethering domain,
• wherein S-C-MT or MT-C-S is configured to be expressed as a single polypeptide, and
• wherein the protease cleavage site comprises
• the amino acid sequence of PRAEYSKGG (SEQ ID NO:
• 181);
• or
• the amino acid sequence of PRAEPIKGG (SEQ ID NO:
• 182);
• or
• the amino acid sequence of PRAEAYKGG (SEQ ID NO:
• 183);
• or
• the amino acid sequence of PRAESSKGG (SEQ ID NO:
• 184);
• or
• the amino acid sequence of PRAEFTKGG (SEQ ID NO:
• 185);
• or
• the amino acid sequence of DEPHYSQRR (SEQ ID NO:
• 187);
• or
• the amino acid sequence of PPLGPIFNPG (SEQ ID NO:
• 188);
• or
• the amino acid sequence of PLAQAYRSS (SEQ ID NO:
• 189);
• or
• the amino acid sequence of TPIDSSFNPD (SEQ ID NO:
• 190);
• or
• the amino acid sequence of VTPEPIFSLI (SEQ ID NO:
• 191).