Animal feed additives comprising a polypeptide having protease activity and uses thereof
AssigneeNovozymes A/S
InventorsLaerke Tvedebrink Haahr, Tine Hoff, Peter Rahbek Oestergaard
Life of this patent
Estimating the term…
Abstract
The present invention relates to animal feed and animal feed additives comprising polypeptides having protease activity, as well as methods of producing proteases, methods of producing animal feed and animal feed additives comprising proteases, and methods of using proteases to improve animal performance and the nutritional value of animal feed.
Claims29
A method comprising administering a polypeptide having protease activity to an animal, said polypeptide selected from the group consisting of:
- (a) polypeptides having at least 85% sequence identity to SEQ ID NO:
- 1;
- and
- (b) polypeptides having at least 85% sequence identity to SEQ ID NO:
- 2,
- wherein said polypeptide is a variant of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, and
- wherein said variant comprises a set of mutations selected from the group consisting of:
- H39D, I43P, N59D, L61Y, S173P, S175P, T297P;
- H39D, N59D, L61P, H83T, S173P, S175P, T297P;
- H39D, N59D, L61P, S173P, S175P, T297P;
- H39D, N59D, L61Y, H83T, S173P, S175P, T297P;
- H39D, N59D, L61Y, S173P, S175P, T297P;
- H39D, N59D, H83T, S173P, S175P, T297P;
- H39D, N59D, E127N, S129M, S173P, S175P, T297P;
- H39D, L61P, S173P, S175P, T297P;
- H39D, L61Y, H83T, S173P, S175P, T297P;
- H39D, L61Y, H123W, V124A, S173P, S175P, T297P;
- H39D, H83T, H123W, V124A, S173P, S175P, T297P;
- H39D, H83T, E127N, S129M, S173P, S175P, T297P;
- I43P, N59D, L61P, H83T, S173P, S175P, T297P;
- I43P, N59D, H123W, V124A, S173P, S175P, T297P;
- I43P, N59D, S173P, S175P, T297P;
- I43P, L61P, H83T, S173P, S175P, T297P;
- I43P, L61P, H123W, V124A, S173P, S175P, T297P;
- I43P, L61P, V124A, R130D, S173P, S175P, T297P;
- I43P, L61P, E127N, S129M, S173P, S175P, T297P;
- I43P, L61P, S173P, S175P, T297P;
- I43P, L61Y, H123W, V124A, S173P, S175P, T297P;
- I43P, L61Y, V124A, R130D, S173P, S175P, T297P;
- I43P, H83T, V124A, R130D, S173P, S175P, T297P;
- N59D, L61P, E127N, S129M, S173P, S175P, T297P;
- N59D, L61Y, H123W, V124A, S173P, S175P, T297P;
- N59D, H83T, H123W, V124A, S173P, S175P, T297P;
- N59D, H83T, S173P, S175P, T297P;
- N59D, E127N, S129M, S173P, S175P, T297P;
- L61P, H83T, E127N, S129M, S173P, S175P, T297P;
- L61P, H83T, S173P, S175P, T297P;
- L61P, H123W, V124A, R130D, S173P, S175P, T297P;
- L61P, V124A, R130D, S173P, S175P, T297P;
- L61P, E127N, S129M, S173P, S175P, T297P;
- L61Y, H83T, E127N, S129M, S173P, S175P, T297P;
- L61Y, H83T, S173P, S175P, T297P;
- L61Y, V124A, R130D, S173P, S175P, T297P;
- L61Y, E127N, S129M, S173P, S175P, T297P;
- H83T, V124A, R130D, S173P, S175P, T297P;
- H83T, E127N, S129M, S173P, S175P, T297P;
- H123W, V124A, R130D, S173P, S175P, T297P;
- and
- S173P, S175P, T297P,
- wherein the positions correspond to the amino acid sequence of SEQ ID NO:
- 2.
A method comprising administering a polypeptide having protease activity to an animal, said polypeptide having at least 90% sequence identity to SEQ ID NO:
- 2 and comprising one or more substitutions selected from the group consisting of S27K, N109K, S111E, S171E, S173P, G174K, S175P, F180Y, G182A, L184F, Q198E, N199K and T297P, wherein the positions correspond to the amino acid sequence of SEQ ID NO:
- 2.
A method comprising administering a polypeptide having protease activity to an animal, said polypeptide having at least 95% sequence identity to SEQ ID NO:
- 2 and comprising one or more substitutions selected from the group consisting of S27K, N109K, S111E, S171E, S173P, G174K, S175P, F180Y, G182A, L184F, Q198E, N199K and T297P, wherein the positions correspond to the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.