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US 12,651,648 B2Granted
Unified platform for rapid production of nanovaccines with enhanced safety, efficacy and precision
AssigneeSanta Clara University
InventorsBiao Lu, Joy Ku, Renceh Flojo, Christopher James Olson
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Abstract
A unified platform including an exosome-based method has been developed that uses only immunogenic epitopes for nanovaccine production. By eliminating these nonessential sequences from the full-length antigen, this new type of nanovaccine is safer as compared to those of existing vaccines. In addition, careful selection of targeting epitopes and incorporation of multiple epitope repeats into nanovaccine will make it more potent and precise than the traditional vaccines.
Claims1
An exosome-based method for producing a nano-vaccine, comprising:
- (a) inputting a full-length antigen sequence as a query in a graphic user interphase-supported software program to search online databases;
- (b) querying the online databases based on the inputted query to identify, retrieve and cluster Major Histocompatibility Class (MHC)-I epitopes, MHC-II epitopes and B-cell epitopes;
- (c) selecting epitope clusters of the MHC-I, the MHC-II and the B-cell epitopes;
- (d) joining and repeating the selected epitope clusters to form a single linear sequence;
- (e) synthesizing and subcloning the single linear sequence into an exosome-targeting scaffold, wherein the exosome-targeting scaffold has five distinctive domains defined as:
- (i) a signal peptide for directing a synthesized protein to an endoplasmic reticulum (ER);
- (ii) a hinge for providing flexibility of the selected epitope clusters;
- iii) a transmembrane helix which dictates exosome-targeting and incorporation, together with shorter flanking sequences;
- (iv) an intraluminal domain for accepting a luminal cargo;
- and
- (v) a reporter for molecular tracking and imaging;
- and
- (f) synthesizing and subcloning the exosome-targeting scaffold into a mammalian expression vector.
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