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US 12,650,588 B2Granted
Multi-view reflector microscope
AssigneeWashington University
InventorsMatthew Lew, Oumeng Zhang
Life of this patent
Estimating the term…
Abstract
An imaging system uses a multi-view reflector (MVR) to simultaneously measure the three-dimensional (3D) position and orientation of a light emitter. The MVR is positioned at a back focal plane (BFP) of a light collecting optical system, and uses pyramid reflectors and/or conical reflectors to reflect light in at least two regions of the BFP to at least two detection channels of a detector. The at least two detection channels produce images that are used to determine the 3D position and orientation of a light emitter. The system may be used, for example, to image the structure of molecules or organelles in a cell, track the movement of molecules in a cell, or to study the interaction of molecules within a cell.
Claims20
An imaging system comprising:
- a multi-view reflector (MVR) configured to split incoming light into at least two beams of light;
- and
- a detector comprising at least two detection elements, wherein the MVR reflects the at least two beams of light in different directions such that each of the at least two beams of light impinge on a separate detection element of the at least two detection elements, the MVR comprises a pyramid reflector and an air pyramid reflector including an aperture face oriented toward incoming light, and a pyramidal face opposite the aperture face, wherein an aperture is defined through the air pyramid reflector through which incoming light may pass.
An imaging system comprising:
- a multi-view reflector (MVR) configured to split incoming light into at least two beams of light;
- and
- a detector comprising at least two detection elements, wherein the MVR reflects the at least two beams of light in different directions such that each of the at least two beams of light impinge on a separate detection element of the at least two detection elements, the MVR comprises a pyramid reflector and a conical reflector having an interior, reflective surface, and a vertex, an aperture being defined therethrough, the conical reflector oriented so that incoming light enters the conical reflector through the aperture in the vertex.
An imaging system comprising:
- a multi-view reflector (MVR) configured to split incoming light into at least two beams of light;
- a first lens positioned between the incoming light and the MVR to direct the incoming light into MVR;
- and
- a detector comprising at least two detection elements, wherein the MVR reflects the at least two beams of light in different directions such that each of the at least two beams of light impinge on a separate detection element of the at least two detection elements, the MVR comprises a pyramid reflector, the pyramid reflector is a glass pyramid, and the glass pyramid is positioned at a back focal plane (BFP) of the first lens.
1 more element
An imaging system comprising:
- a multi-view reflector (MVR) configured to split incoming light into at least two beams of light;
- and
- a detector comprising at least two detection elements, wherein the MVR reflects the at least two beams of light in different directions such that each of the at least two beams of light impinge on a separate detection element of the at least two detection elements, the at least two detection elements comprise a charge coupled device (CCD) camera, a complementary metal-oxide-semiconductor (CMOS) camera, a photomultiplier tube, or an avalanche photodiode, and the at least two detection elements are provided by a single detection element having two or more channels, and each of the two or more channels is a different one of the at least two detection elements.
An imaging system comprising:
- a detector including at least two detection elements;
- a multi-view reflector (MVR) including an air pyramid reflector and a glass pyramid reflector positioned between incoming light and the detector, the air pyramid reflector including an aperture face, a pyramidal face opposite the aperture face, and an aperture from the aperture face to the pyramidal face, the aperture face being oriented toward incoming light, and the glass pyramid reflector is positioned with an apex of the glass pyramid reflector pointing towards the aperture on the pyramidal face side of the air pyramid reflector to split incoming light into at least two beams of light and reflect the at least two beams of light to the pyramidal face of the air pyramid reflector for reflection by the air pyramid reflector to the detector;
- a first lens positioned to receive incoming light and configured to direct the incoming light through the aperture of the air pyramid reflector to the glass pyramid reflector;
- and
- a second lens positioned to receive the at least two light beams reflected from the air pyramid reflector and configured to direct the at least two beams of light to the detector.
2 more elements
An imaging system comprising:
- a detector including at least two detection elements;
- a multi-view reflector (MVR) including a conical reflector and a glass pyramid reflector positioned between incoming light and the detector;
- the conical reflector including an exterior surface, an interior, reflective surface, and a vertex having an aperture from the exterior surface of the cone to the interior, reflective surface;
- the conical reflector being oriented so that incoming light enters the conical reflector through the aperture, and the glass pyramid reflector is positioned with an apex of the glass pyramid reflector pointing towards the aperture on the interior reflective surface side of the conical reflector to split incoming light into at least two beams of light and reflect the at least two beams of light to the interior, reflective surface of the conical reflector for reflection by the conical reflector to the detector;
- a first lens positioned to receive incoming light and configured to direct the incoming light through the aperture of the conical reflector to the glass pyramid reflector;
- and
- a second lens positioned to receive the at least two light beams reflected from the conical reflector and configured to direct the at least two beams of light to the detector.
4 more elements
Dependencies are inferred from the claim text.